Dynactin is required for bidirectional organelle transport

Sean W. Deacon, Anna S. Serpinskaya, Patricia S. Vaughan, Monica Lopez Fanarraga, Isabelle Vernos, Kevin T. Vaughan, Vladimir I. Gelfand*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

262 Scopus citations


Kinesin II is a heterotrimeric plus end-directed microtubule motor responsible for the anterograde movement of organelles in various cell types. Despite substantial literature concerning the types of organelles that kinesin II transports, the question of how this motor associates with cargo organelles remains unanswered. To address this question, we have used Xenopus laevis melanophores as a model system. Through analysis of kinesin II-mediated melanosome motility, we have determined that the dynactin complex, known as an anchor for cytoplasmic dynein, also links kinesin II to organelles. Biochemical data demonstrates that the putative cargo-binding subunit of Xenopus kinesin II, Xenopus kinesin II-associated protein (XKAP), binds directly to the p150Glued subunit of dynactin. This interaction occurs through aa 530-793 of XKAP and aa 600-811 of p150Glued. These results reveal that dynactin is required for transport activity of microtubule motors of opposite polarity, cytoplasmic dynein and kinesin II, and may provide a new mechanism to coordinate their activities.

Original languageEnglish (US)
Pages (from-to)297-301
Number of pages5
JournalJournal of Cell Biology
Issue number3
StatePublished - Feb 3 2003


  • Dynactin
  • Dynein
  • Kinesin II
  • Melanophores
  • Organelle transport

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Dynactin is required for bidirectional organelle transport'. Together they form a unique fingerprint.

Cite this