Dynamic aspects of intermediate filament networks in BHK-21 cells

K. L. Vikstrom, G. G. Borisy, R. D. Goldman

Research output: Contribution to journalArticlepeer-review

120 Scopus citations


A procedure was developed for the conjugation of vimentin with biotin. Biotinylated vimentin was then microinjected into BHK-21 cells and the fate of the labeled protein was determined at various times postinjection by indirect immunofluorescence. Microinjected vimentin could be traced through a specific sequence of morphological changes ultimately resulting in the formation of a filamentous network. The injected protein was first detected in spots dispersed throughout the cytoplasm. Subsequently, these spots appeared to cluster near the nucleus where they merged into a diffuse 'cap'. This cap coincided with a concentration of endogenous intermediate filaments and eventually gave rise to a filamentous network that was coincident with the endogenous intermediate filament network as a determined by double-label immunofluorescence. The results indicate that the incorporation of exogenous vimentin into a filamentous network is initiated in a perinuclear region and progresses in a polarized fashion toward the cell surface.

Original languageEnglish (US)
Pages (from-to)549-553
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number2
StatePublished - 1989

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Dynamic aspects of intermediate filament networks in BHK-21 cells'. Together they form a unique fingerprint.

Cite this