Dynamic development of the calyx of Held synapse

Adrián Rodríguez-Contreras*, John Silvio Soria Van Hoeve, Ron L.P. Habets, Heiko Locher, J. Gerard G. Borst

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

The calyx of Held is probably the largest synaptic terminal in the brain, forming a unique one-to-one connection in the auditory ventral brainstem. During early development, calyces have many collaterals, whose function is unknown. Using electrophysiological recordings and fast-calcium imaging in brain slices, we demonstrate that these collaterals are involved in synaptic transmission. We show evidence that the collaterals are pruned and that the pruning already begins 1 week before the onset of hearing. Using two-photon microscopy to image the calyx of Held in neonate rats, we report evidence that both axons and nascent calyces are structurally dynamic, showing the formation, elimination, extension, or retraction of up to 65% of their collaterals within 1 hour. The observed dynamic behavior of axons may add flexibility in the choice of postsynaptic partners and thereby contribute to ensuring that each principal cell eventually is contacted by a single calyx of Held.

Original languageEnglish (US)
Pages (from-to)5603-5608
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number14
DOIs
StatePublished - Apr 8 2008

Keywords

  • Auditory system
  • Axon collateral
  • Medial nucleus of the trapezoid body
  • Structural plasticity
  • Two-photon imaging

ASJC Scopus subject areas

  • General

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