Dynamic monitoring of salmonella typhimurium infection of polarized epithelia using organic transistors

Scherrine A. Tria, Marc Ramuz, Miriam Huerta, Pierre Leleux, Jonathan Rivnay, Leslie H. Jimison, Adel Hama, George G. Malliaras, Róisín M. Owens*

*Corresponding author for this work

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Ion flow across polarized epithelia is a tightly regulated process. Measurement of the transepithelial resistance is a highly relevant parameter for assessing the function or health of the tissue. Dynamic, electrical measurements of transepithelial ion flow are preferred as they provide the most accurate snapshot of effects of external stimuli. Enteric pathogens such as Salmonella typhimurium are known to disrupt ion flow in gastrointestinal epithelia. Here, for the first time, the use of organic transistors as a powerful potential alternative for front-line, disposable, high-throughput diagnostics of enteric pathogens is demonstrated. The transistors' ability to detect early and subtle changes in transepithelial ion flow is capitalized upon to develop a highly sensitive detector of epithelial integrity. Stable operation of the organic devices under physiological conditions is shown, followed by dynamic, pathogen-specific diagnosis of infection of epithelia. Further, operation of the device is possible in complex matrices, showing particular promise for food and safety applications.

Original languageEnglish (US)
Pages (from-to)1053-1060
Number of pages8
JournalAdvanced Healthcare Materials
Volume3
Issue number7
DOIs
StatePublished - Jul 2014

Keywords

  • Diagnostics
  • Epithelial tissue
  • Organic electronics
  • Salmonella typhimurium
  • Transepithelial resistance

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

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