Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein

Yue Jia, Ka Wing Fong, Yuk Kwan Choi, Siu San See, Robert Z. Qi

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

CDK5RAP2 is a centrosomal protein known to be involved in the regulation of the γ-tubulin ring complex and thus the organization of microtubule arrays. However, the mechanism by which CDK5RAP2 is itself recruited to centrosomes is poorly understood. We report here that CDK5RAP2 displays highly dynamic attachment to centrosomes in a microtubule-dependent manner. CDK5RAP2 associates with the retrograde transporter dynein-dynactin and contains a sequence motif that binds to dynein light chain 8. Significantly, disruption of cellular dynein-dynactin function reduces the centrosomal level of CDK5RAP2. These results reveal a key role of the dynein-dynactin complex in the dynamic recruitment of CDK5RAP2 to centrosomes.

Original languageEnglish (US)
Article numbere68523
JournalPloS one
Volume8
Issue number7
DOIs
StatePublished - Jul 17 2013

ASJC Scopus subject areas

  • General
  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences

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