Dynamic transcriptional events in embryonic stem cells mediated by the super elongation complex (SEC)

Chengqi Lin, Alexander S. Garrett, Bony de Kumar, Edwin R. Smith, Madelaine Gogol, Christopher Seidel, Robb Krumlauf, Ali Shilatifard*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

Transcriptional regulation of developmentally controlled genes is at the heart of differentiation and organogenesis. In this study, we performed global genomic analyses in murine embryonic stem (ES) cells and in human cells in response to activation signals. We identified an essential role for the ELL (eleven-nineteen lysine-rich leukemia gene)/P-TEFb (positive transcription elongation factor)-containing super elongation complex (SEC) in the regulation of gene expression, including several genes bearing paused RNA polymerase II (Pol II). Paused Pol II has been proposed to be associated with loci that respond rapidly to environmental stimuli. However, our studies in ES cells also identified a requirement for SEC at genes without paused Pol II, which also respond dynamically to differentiation signals. Our findings suggest that SEC is a major class of active P-TEFb-containing complexes required for transcriptional activation in response to environmental cues such as differentiation signals.

Original languageEnglish (US)
Pages (from-to)1486-1498
Number of pages13
JournalGenes and Development
Volume25
Issue number14
DOIs
StatePublished - Jul 15 2011

Keywords

  • AFF4
  • ELL2
  • Immediate early genes
  • Transcription elongation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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