Dynamics of the human infectious reservoir for malaria determined by mosquito feeding assays and ultrasensitive malaria diagnosis in Burkina Faso

André Lin Ouédraogo*, Bronner P. Gonçalves, Awa Gnémé, Edward A. Wenger, Moussa W. Guelbeogo, Amathe Ouédraogo, Jaline Li Gerardin, Caitlin A. Bever, Hil Lyons, Xavier Pitroipa, Jan Peter Verhave, Philip A. Eckhoff, Chris Drakeley, Robert Sauerwein, Adrian J.F. Luty, Bocar Kouyaté, Teun Bousema

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Background Plasmodium falciparum gametocytes are essential for malaria transmission. Malaria control measures that aim at reducing transmission require an accurate characterization of the human infectious reservoir. Methods We longitudinally determined human infectiousness to mosquitoes and P. falciparum carriage by an ultrasensitive RNA-based diagnostics in 130 randomly selected inhabitants of an endemic area. Results At least 1 mosquito was infected by 32.6% (100 of 307) of the blood samples; in total, 7.6% of mosquitoes (916 of 12 079) were infected. The proportion of infectious individuals and infected mosquitoes were negatively associated with age and positively with asexual parasites (P <. 001). Human infectiousness was higher at the start of the wet season and subsequently declined at the peak of the wet season (adjusted odds ratio, 0.52; P =. 06) and in the dry season (0.23; P <. 001). Overall, microscopy-negative individuals were responsible for 28.7% of infectious individuals (25 of 87) and 17.0% of mosquito infections (145 of 855). Conclusions Our study reveals that the infectious reservoir peaks at the start of the wet season, with prominent roles for infections in children and submicroscopic infections. These findings have important consequences for strategies and the timing of interventions, which need to include submicroscopic infections and be implemented in the dry season.

Original languageEnglish (US)
Pages (from-to)90-99
Number of pages10
JournalJournal of Infectious Diseases
Volume213
Issue number1
DOIs
StatePublished - Jan 1 2016

Keywords

  • malaria; falciparum; gametocyte; reservoir; season; dynamics; QT-NASBA; mosquito; feeding; Burkina Faso

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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