TY - JOUR
T1 - Dysregulation of temperature and liver cytokine gene expression in immunodeficient wasted mice
AU - Libertin, Claudia R.
AU - Ling-Indeck, Lydia
AU - Weaver, Paul
AU - Chang-Liu, Chin Mei
AU - Strezoska, Vesna
AU - Heckert, Barbara
AU - Woloschak, Gayle E.
N1 - Funding Information:
We gratefully acknowledge the following individuals who provided us with cDNA clones: α-fetoprotein from S. Tilghman (Princeton University, Princeton, NJ); pHRR rRNA from E. Wieben (Mayo Clinic, MN); Rp-8 from G. P. Owens (University of Colorado, Denver, CO); ornithine amino transferase from C. Peraino (Argonne National Laboratory); C-reactive protein from Dr. S. B. Dowton (Washington University); PCNA from R. Brava (Bristol Myers); and IL1, IL6, c-jun, and c-fos from American Type Culture Collection (Rockville, MD). The authors thank Kay Bexson for excellent secretarial assistance and Drs. Cheryl Denault, Frank Collart, and Tom Seed for critical review of the manuscript. Work supported by the U.S. Department of Energy, Of®ce of Health and Environmental Research, under Contract No. W-31-109-ENG-38.
PY - 1996/4/10
Y1 - 1996/4/10
N2 - Wasted mice bear the spontaneous autosomal recessive mutation wst/wst; this genotype is associated with weight loss beginning at 21 days of ape, neurologic dysfunction, immunodeficiency at mucosal sites, and increased sensitivity to the killing effects of ionizing radiation. The pathology underlying the disease symptoms is unknown. Experiments reported here were designed to examine thermoregulation and liver expression of specific cytokines in wasted mice and in littermate and parental controls. Our experiments found that wasted mice begin to show a drop in body temperature at 21-23 days following birth, continuing until death at the age of 28 days. Concomitant with that, livers from wasted mice expressed increased amounts of mRNAs specific for cytokines IL-6 and IL-1, the acute phase reactant C reactive protein, c-jun, and apoptosis-associated Rp-8 when compared to littermate and parental control animals. Levels of transforming growth factor, c-fos, proliferating cell nuclear antigen, and ornithine amino transferase transcripts were the same in livers from wasted mice and controls. These results suggest a relationship between an acute phase reactant response in wasted mice and temperature dysregulation.
AB - Wasted mice bear the spontaneous autosomal recessive mutation wst/wst; this genotype is associated with weight loss beginning at 21 days of ape, neurologic dysfunction, immunodeficiency at mucosal sites, and increased sensitivity to the killing effects of ionizing radiation. The pathology underlying the disease symptoms is unknown. Experiments reported here were designed to examine thermoregulation and liver expression of specific cytokines in wasted mice and in littermate and parental controls. Our experiments found that wasted mice begin to show a drop in body temperature at 21-23 days following birth, continuing until death at the age of 28 days. Concomitant with that, livers from wasted mice expressed increased amounts of mRNAs specific for cytokines IL-6 and IL-1, the acute phase reactant C reactive protein, c-jun, and apoptosis-associated Rp-8 when compared to littermate and parental control animals. Levels of transforming growth factor, c-fos, proliferating cell nuclear antigen, and ornithine amino transferase transcripts were the same in livers from wasted mice and controls. These results suggest a relationship between an acute phase reactant response in wasted mice and temperature dysregulation.
UR - http://www.scopus.com/inward/record.url?scp=0029863993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029863993&partnerID=8YFLogxK
U2 - 10.1006/cimm.1996.0091
DO - 10.1006/cimm.1996.0091
M3 - Article
C2 - 8612295
AN - SCOPUS:0029863993
VL - 169
SP - 62
EP - 66
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -