Early- and intermediate-stage variants of simian immunodeficiency virus replicate efficiently in cells lacking CCR5

Serene Forte, Mary Elizabeth Harmon, Mario J. Pineda, Julie Overbaugh*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Primate lentiviruses are thought to use the chemokine receptor CCR5 as the major coreceptor for entry into cells. Here we show that some variants of simian immunodeficiency virus (SIV) replicate efficiently in peripheral blood mononuclear cells (PBMCs) lacking a functional CCR5. There were differences in the replication patterns of sequential variants that evolved during SIVMne infection; the late-stage pathogenic variants were unable to replicate in PBMCs lacking CCRS, whereas the early- and intermediate-stage viruses replicated as well in PBMCs lacking CCR5 as they did in cells with wild-type CCR5. The coreceptor specificities of these sequential variants were compared using indicator cell lines expressing known SIV coreceptors. Among the known SIV coreceptors, there were none that were functional for the early and intermediate variants but not the late-stage variants, suggesting that the coreceptor used for replication in PBMCs may be a coreceptor that has not yet been described. Because some variants replicate with high efficiency in peripheral blood cells using this as yet uncharacterized cellular receptor, this coreceptor may be important for viral entry of some target cell populations in the host.

Original languageEnglish (US)
Pages (from-to)9723-9727
Number of pages5
JournalJournal of virology
Volume77
Issue number17
DOIs
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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