Early growth response-2 expression in uterine leiomyoma cells: Regulation and function

Ping Yin, Antonia Navarro, Feng Fang, Anna Xie, John S. Coon, Carrie Richardson, Serdar E. Bulun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Objective: To investigate the regulation of early growth response-2 (Egr-2) by transforming growth factor β3 (TGF-β3) and its functions in cultured human uterine leiomyoma smooth muscle cells. Design: Laboratory research. Setting: Academic medical center. Patient(s): Primary leiomyoma cells from patients with symptomatic leiomyomata. Intervention(s): Tissue culture followed by RNA and protein analysis. Main Outcome Measure(s): Cell proliferation, alteration in extracellular matrix component expression. Result(s): In vivo mRNA levels of Egr-2 were statistically significantly higher in leiomyoma tissues compared with matched myometrial tissues, and showed a statistically significant correlation with TGF-β3 messenger RNA (mRNA) levels in leiomyoma tissues. In primary leiomyoma smooth muscle cells, TGF-β3 statistically significantly induced Egr-2 gene expression in a dose-dependent and time-dependent manner. Small interfering RNA (siRNA) knockdown of Egr-2 markedly increased the level of the proliferation marker proliferating cell nuclear antigen and the expression of proto-oncogene c-myc. On the other hand, ablation of Egr-2 stimulated collagen-1A1 and collagen-3A1 transcription and inhibited dermatopontin gene expression. However, the mRNA levels of α-smooth muscle actin and fibronectin were not affected by Egr-2 knockdown. Conclusion(s): We demonstrated that TGF-β3 regulated Egr-2 gene expression and presented evidence that Egr-2 decreases collagen production and stimulates dermatopontin gene expression.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalFertility and Sterility
Volume96
Issue number2
DOIs
StatePublished - Aug 2011

Keywords

  • Collagen
  • Egr-2
  • PCNA
  • TGF-β3
  • c-myc
  • dermatopontin
  • leiomyomata

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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