Early histopathology of small intestinal discordant xenografts

Emre N. Yedidag, Jonathan P. Fryer, Edi Levi, Francis C. Buckingham, David Ivancic, Jeremy Kraff, Cheng Fang Huang, Alfred W. Rademaker, Dixon B. Kaufman, Michael Abecassis, Frank P. Stuart*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A descriptive study of a new model enabling serial biopsies of ongoing hyperacute rejection of small intestinal discordant xenografts is presented. In a series of guinea-pig-to-Lewis rat small bowel xenotransplants (n=7), aboral free ends of Thierry-Vella loops constructed from the graft were sequentially biopsied at one-minute intervals up to ten minutes post- reperfusion and less frequently thereafter. In a guinea pig-to-guinea pig (n=6) isograft series, biopsy controls for preservation/ischemia-reperfusion injury were obtained. Xenoantibody sequestration in this model was evaluated in a separate series of transplants, utilizing an ELISA assay for rat anti- guinea pig natural antibodies. Pathologic evaluation revealed a unique series of events characterized with microcirculatory failure and thrombosis progressing from the submucosal vasculature to the lumen. Within the system's detection limits, complement deposition and P-selectin expression occurred as early as one minute post-reperfusion, preceding the staining for IgM and IgG. Using rat serum ELISAs, no significant difference in xenoantibody sequestration was detected between the xenograft and isograft groups. The guinea pig-to-rat discordant small bowel xenotransplantation is an efficient small animal model to dissect the very early pathophysiologic events during hyperacute rejection.

Original languageEnglish (US)
Pages (from-to)1385-1391
Number of pages7
JournalTransplantation
Volume62
Issue number10
DOIs
StatePublished - Nov 27 1996

ASJC Scopus subject areas

  • Transplantation

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