Early in vivo and in vitro effects of amelogenin gene splice products on pulp cells

Sally Lacerda-Pinheiro, Nadege Jegat, Dominique Septier, Fabienne Priam, Mireille Bonnefoix, Juliette Bitard, Odile Kellermann, Kevin Tompkins, Arthur Veis, Michel Goldberg*, Anne Poliard

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Recombinant amelogenin gene splice products A+4 and A-4, implanted in the pulp, induce the recruitment, proliferation, and differentiation of reparative cells. Our aim was to investigate the precocious events occurring in the pulp 1 d and 3 d after implantation of agarose beads alone or loaded with A+4 or A-4. Proliferation and cell recruitment towards an odonto/osteogenic phenotype were visualized by detection of the proliferation cell nuclear antigen (PCNA) and RP59. After implantation of beads alone or loaded with A+4, at day 3, pulp cells were moderately immunopositive for osteopontin (OP), whereas labeling was strongly positive upon treatment with A-4. Dentin sialoprotein (DSP) labeling was not detectable. Parallel in vitro studies were carried out on odontoblastic and mesenchymal progenitor cells in order to evaluate the effect of the amelogenin peptides on the expression of a series of marker genes involved in the odontoblastic/osteogenic/chondrogenic differentiation pathways. Altogether, our results suggest that the 'signaling' effects of the amelogenin peptides A+4 and A-4 may differ according to the type of target cells, their stage of differentiation, the time of treatment, and the type of amelogenin peptide (A+4 or A-4).

Original languageEnglish (US)
Pages (from-to)232-238
Number of pages7
JournalEuropean Journal of Oral Sciences
Issue numberSUPPL. 1
StatePublished - May 1 2006


  • Amelogenin gene splice product
  • Exposed pulp
  • Osteoblast/odontoblast progenitors

ASJC Scopus subject areas

  • Dentistry(all)


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