Early-life intranasal colonization with nontypeable Haemophilus influenzae exacerbates juvenile airway disease in mice

Jessica R. McCann*, Stanley N. Mason, Richard L. Auten, Joseph W. St. Geme, Patrick C. Seed

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Accumulating evidence suggests a connection between asthma development and colonization with nontypeable Haemophilus influenzae (NTHi). Specifically, nasopharyngeal colonization of human infants with NTHi within 4 weeks of birth is associated with an increased risk of asthma development later in childhood. Monocytes derived from these infants have aberrant inflammatory responses to common upper respiratory bacterial antigens compared to those of cells derived from infants who were not colonized and do not go on to develop asthma symptoms in childhood. In this study, we hypothesized that early-life colonization with NTHi promotes immune system reprogramming and the development of atypical inflammatory responses. To address this hypothesis in a highly controlled model, we tested whether colonization of mice with NTHi on day of life 3 induced or exacerbated juvenile airway disease using an ovalbumin (OVA) allergy model of asthma. We found that animals that were colonized on day of life 3 and subjected to induction of allergy had exacerbated airway disease as juveniles, in which exacerbated airway disease was defined as increased cellular infiltration into the lung, increased amounts of inflammatory cytokines interleukin-5 (IL-5) and IL-13 in lung lavage fluid, decreased regulatory T cell-associated FOXP3 gene expression, and increased mucus production. We also found that colonization with NTHi amplified airway resistance in response to increasing doses of a bronchoconstrictor following OVA immunization and challenge. Together, the murine model provides evidence for early-life immune programming that precedes the development of juvenile airway disease and corroborates observations that have been made in human children.

Original languageEnglish (US)
Pages (from-to)2022-2030
Number of pages9
JournalInfection and immunity
Volume84
Issue number7
DOIs
StatePublished - Jan 1 2016

Fingerprint

Haemophilus influenzae
Asthma
Ovalbumin
Hypersensitivity
Bronchoconstrictor Agents
Bacterial Antigens
Airway Resistance
Interleukin-13
Interleukin-5
Bronchoalveolar Lavage Fluid
Regulatory T-Lymphocytes
Mucus
Monocytes
Immune System
Immunization
Parturition
Cytokines
Gene Expression
Lung

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

McCann, Jessica R. ; Mason, Stanley N. ; Auten, Richard L. ; St. Geme, Joseph W. ; Seed, Patrick C. / Early-life intranasal colonization with nontypeable Haemophilus influenzae exacerbates juvenile airway disease in mice. In: Infection and immunity. 2016 ; Vol. 84, No. 7. pp. 2022-2030.
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Early-life intranasal colonization with nontypeable Haemophilus influenzae exacerbates juvenile airway disease in mice. / McCann, Jessica R.; Mason, Stanley N.; Auten, Richard L.; St. Geme, Joseph W.; Seed, Patrick C.

In: Infection and immunity, Vol. 84, No. 7, 01.01.2016, p. 2022-2030.

Research output: Contribution to journalArticle

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AU - Mason, Stanley N.

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AU - Seed, Patrick C.

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