Early role of Fsp1 in epithelial-mesenchymal transformation

Hirokazu Okada, Theodore M. Danoff, Raghuram Kalluri, Eric G. Neilson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

394 Scopus citations

Abstract

A seamless plasticity exists among cells shifting between epithelial and mesenchymal phenotypes during early development and again later, in adult tissues, following wound repair or organ remodeling in response to injury. Fsp1, a gene encoding a fibroblast-specific protein associated with mesenchymal-cell morphology and motility, is expressed during epithelial- mesenchymal transformations (EMT) in vivo. In the current study, we identified several cytokines that induce Fsp1 in cultured epithelial cells. A combination of these factors, however, was most efficacious at completing the process of EMT. The optimal combination identified were two of the cytokines classically associated with fibrosis, i.e., transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF). To confirm that it was the induction of Fsp1 by these cytokines mediating EMT, we used antisense oligomers to block Fsp1 production and subsequently measured cell motility and markers of EMT phenotype. The antisense oligomers suppressed Fsp1 expression and epithelial transformation; therefore, we conclude that the appearance of Fsp1 is an important early event in the pathway toward EMT.

Original languageEnglish (US)
Pages (from-to)F563-F574
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number4 42-4
DOIs
StatePublished - Oct 1997

Keywords

  • Antisense
  • Cell motility
  • Epidermal growth factor
  • Fibroblast
  • Fibrosis
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Physiology
  • Urology

Fingerprint Dive into the research topics of 'Early role of Fsp1 in epithelial-mesenchymal transformation'. Together they form a unique fingerprint.

Cite this