Early supersensitive changes in [3H]spiroderidol binding in the isolated striatum

John G. Csernansky*, Arnold J. Friedhoff, Kenneth A. Bonnet

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Adult mouse striatum was dissected and incubated as an organ culture in chemical medium for up to 24 hours to measure alterations in [3H]spiroperidol binding. High-affinity [3H]spiroperidol binding (ligand concentration 2 nM) was constant throughout the incubation. However, there was a 33% increase in lower-affinity binding (ligand concentration 20 nM) during 24 hours of incubation. Inhibition of dopamine synthesis with alpha-methyl-p-tyrosine methyl ester enhanced the increase in 20 nM [3H]spiroperidol binding by 65%.

Original languageEnglish (US)
Pages (from-to)117-120
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume105
Issue number1
DOIs
StatePublished - Mar 15 1982

Fingerprint

Spiperone
Ligands
Organ Culture Techniques
Dopamine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "Adult mouse striatum was dissected and incubated as an organ culture in chemical medium for up to 24 hours to measure alterations in [3H]spiroperidol binding. High-affinity [3H]spiroperidol binding (ligand concentration 2 nM) was constant throughout the incubation. However, there was a 33{\%} increase in lower-affinity binding (ligand concentration 20 nM) during 24 hours of incubation. Inhibition of dopamine synthesis with alpha-methyl-p-tyrosine methyl ester enhanced the increase in 20 nM [3H]spiroperidol binding by 65{\%}.",
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Early supersensitive changes in [3H]spiroderidol binding in the isolated striatum. / Csernansky, John G.; Friedhoff, Arnold J.; Bonnet, Kenneth A.

In: Biochemical and Biophysical Research Communications, Vol. 105, No. 1, 15.03.1982, p. 117-120.

Research output: Contribution to journalArticle

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