Adult mouse striatum was dissected and incubated as an organ culture in chemical medium for up to 24 hours to measure alterations in [3H]spiroperidol binding. High-affinity [3H]spiroperidol binding (ligand concentration 2 nM) was constant throughout the incubation. However, there was a 33% increase in lower-affinity binding (ligand concentration 20 nM) during 24 hours of incubation. Inhibition of dopamine synthesis with alpha-methyl-p-tyrosine methyl ester enhanced the increase in 20 nM [3H]spiroperidol binding by 65%.
|Original language||English (US)|
|Number of pages||4|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 15 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology