Early supersensitive changes in [³H]spiroderidol binding in the isolated striatum

Adult mouse striatum was dissected and incubated as an organ culture in chemical medium for up to 24 hours to measure alterations in [³H]spiroperidol binding. High-affinity [³H]spiroperidol binding (ligand concentration 2 nM) was constant throughout the incubation. However, there was a 33% increase in lower-affinity binding (ligand concentration 20 nM) during 24 hours of incubation. Inhibition of dopamine synthesis with alpha-methyl-p-tyrosine methyl ester enhanced the increase in 20 nM [³H]spiroperidol binding by 65%.