Abstract
Adult mouse striatum was dissected and incubated as an organ culture in chemical medium for up to 24 hours to measure alterations in [3H]spiroperidol binding. High-affinity [3H]spiroperidol binding (ligand concentration 2 nM) was constant throughout the incubation. However, there was a 33% increase in lower-affinity binding (ligand concentration 20 nM) during 24 hours of incubation. Inhibition of dopamine synthesis with alpha-methyl-p-tyrosine methyl ester enhanced the increase in 20 nM [3H]spiroperidol binding by 65%.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 117-120 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 105 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 15 1982 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Early supersensitive changes in [3H]spiroderidol binding in the isolated striatum. / Csernansky, John G.; Friedhoff, Arnold J.; Bonnet, Kenneth A.
In: Biochemical and Biophysical Research Communications, Vol. 105, No. 1, 15.03.1982, p. 117-120.Research output: Contribution to journal › Article
TY - JOUR
T1 - Early supersensitive changes in [3H]spiroderidol binding in the isolated striatum
AU - Csernansky, John G.
AU - Friedhoff, Arnold J.
AU - Bonnet, Kenneth A.
PY - 1982/3/15
Y1 - 1982/3/15
N2 - Adult mouse striatum was dissected and incubated as an organ culture in chemical medium for up to 24 hours to measure alterations in [3H]spiroperidol binding. High-affinity [3H]spiroperidol binding (ligand concentration 2 nM) was constant throughout the incubation. However, there was a 33% increase in lower-affinity binding (ligand concentration 20 nM) during 24 hours of incubation. Inhibition of dopamine synthesis with alpha-methyl-p-tyrosine methyl ester enhanced the increase in 20 nM [3H]spiroperidol binding by 65%.
AB - Adult mouse striatum was dissected and incubated as an organ culture in chemical medium for up to 24 hours to measure alterations in [3H]spiroperidol binding. High-affinity [3H]spiroperidol binding (ligand concentration 2 nM) was constant throughout the incubation. However, there was a 33% increase in lower-affinity binding (ligand concentration 20 nM) during 24 hours of incubation. Inhibition of dopamine synthesis with alpha-methyl-p-tyrosine methyl ester enhanced the increase in 20 nM [3H]spiroperidol binding by 65%.
UR - http://www.scopus.com/inward/record.url?scp=0020421678&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020421678&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(82)80018-1
DO - 10.1016/S0006-291X(82)80018-1
M3 - Article
C2 - 6124243
AN - SCOPUS:0020421678
VL - 105
SP - 117
EP - 120
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -