EEDA: A protein associated with an early stage of stratified epithelial differentiation

Lijie Sun, David G. Ryan, Mingyuan Zhou, Tung Tien Sun, Robert M. Lavker*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Using suppressive subtractive hybridization, we have identified a novel gene, which we named early epithelial differentiation associated (EEDA), which is uniquely associated with an early stage of stratified epithelial differentiation. In epidermis, esophageal epithelium, and tongue epithelium, EEDA mRNA, and antigen was abundant in suprabasal cells, but was barely detectable in more differentiated cells. Consistent with the limbal location of corneal epithelial stem cells, EEDA was expressed in basal corneal epithelial cells that are out of the stem cell compartment, as well as the suprabasal corneal epithelial cells. The strongest EEDA expression occurred in suprabasal precortical cells of mouse, bovine, and human anagen follicles. Developmental studies showed that the appearance of EEDA in embryonic mouse epidermis (E 15.5) coincided with morphological keratinization. Interestingly, EEDA expression is turned off when epithelia were perturbed by wounding and by cultivation under both low and high Ca2+ conditions. Our results indicate that EEDA is involved in the early stages of normal epithelial differentiation, and that EEDA is important for the "normal" differentiation pathway in a wide range of stratified epithelia.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalJournal of Cellular Physiology
Issue number1
StatePublished - Jan 2006

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


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