2-Deoxy-d-glucose (2dG) and glucosamine reversibly inhibited the replication and cytopathic effect of two viruses pathogenic for man, respiratory syncytial virus and parainfluenza type 3, by affecting a late step in the growth cycle. Functions attributed to viral glycoproteins, including hemadsorption, hemagglutination, and cell fusion, were significantly depressed whereas complement fixation (CF) antigen(s) was largely unaffected. Viral surface antigens were demonstrable in the cytoplasm but could not be detected on the surface of infected cells treated with these inhibitors. Reversal of the block produced by the inhibitors was rapid and dependent on new protein synthesis. The inhibitory effects of 2dG and glucosamine were distinguishable by the course of their reversal as well as the observation that mannose specifically reversed and prevented only the 2dG inhibition.
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