Effect of corynebacterium parvum, methanol-extraction residue of BCG, and levamisole on macrophage random Migration, Chemotaxis, and Pinocytosis

Neal A. Sher; David G. Poplack; R. Michael Blaese; Teresa M. Brown; Sotiros D. Chaparas

doi:10.1093/jnci/58.6.1753

Effect of corynebacterium parvum, methanol-extraction residue of BCG, and levamisole on macrophage random Migration, Chemotaxis, and Pinocytosis

Neal A. Sher^*, David G. Poplack, R. Michael Blaese, Teresa M. Brown, Sotiros D. Chaparas

^*Corresponding author for this work

Ophthalmology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Three parameters of macrophage function: Random migration, chemotaxis, and pinocytosis, were studied in the guinea pig after administration of Corynebacterlum parvum, methanol-extraction residue of BCG, and levamisole (LMS), a synthetic anthelmintic. Macrophage migration studies were performed with a modified Boyden chamber. Pinocytosis was assessed by the uptake of colloidal¹⁹⁸Au. After ip administration, each of the three immunostimulators induced an increase in macrophage chemotactic responsiveness and, to a lesser extent and duration, in random motility. Kinetic, dose-response, and time course data for the effect of each agent on macrophage movement were explored. LMS was the most effective stimulator of macrophage activation, which occurred earlier and persisted longer than it did with the other agents. Macrophages from animals receiving each of the agents showed enhanced pinocytosis. Measurement of macrophage random migration, chemotaxis, and pinocytosis appeared to provide a rapid and quantitative assessment of several parameters of macrophage function and, when studied with other immunologic parameters, may provide useful tools for the evaluation of potential immunoadjuvants.

Original language	English (US)
Pages (from-to)	1753-1757
Number of pages	5
Journal	Journal of the National Cancer Institute
Volume	58
Issue number	6
DOIs	https://doi.org/10.1093/jnci/58.6.1753
State	Published - Jun 1977

Funding

ABBREVIATIONS USED: MER = methanol-extraction residue of BCG; LMS = levamisole; SSV = steroid suspending vehicle; PEC = peritoneal exudate cells; GBBS = Gey's balanced salt solution; cpm = counts per minute. 1 Received August 25, 1976; accepted January 5, 1977. 2 Mycobacterial and Fungal Antigen Branch, Division of Bacterial Products, Bureau of Biologics, U.S. Food and Drug Administration, Bethesda, Md. 20014. 3 Present address: Department of Ophthalmology, University of Minnesota School of Medicine, Mayo Memorial Bldg., Box 449, Minneapolis, Minn. 55455. 4 Pediatric Oncology Branch, Division of Cancer Treatment, National Cancer Institute (NCI), National Institutes of Health, Public Health Service, U. S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. 5 Metabolism Branch, Division of Cancer Biology and Diagnosis, NCI.

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/jnci/58.6.1753

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@article{19db23c81d4c4e7e81ec2a933ad7f9c6,

title = "Effect of corynebacterium parvum, methanol-extraction residue of BCG, and levamisole on macrophage random Migration, Chemotaxis, and Pinocytosis",

abstract = "Three parameters of macrophage function: Random migration, chemotaxis, and pinocytosis, were studied in the guinea pig after administration of Corynebacterlum parvum, methanol-extraction residue of BCG, and levamisole (LMS), a synthetic anthelmintic. Macrophage migration studies were performed with a modified Boyden chamber. Pinocytosis was assessed by the uptake of colloidal198Au. After ip administration, each of the three immunostimulators induced an increase in macrophage chemotactic responsiveness and, to a lesser extent and duration, in random motility. Kinetic, dose-response, and time course data for the effect of each agent on macrophage movement were explored. LMS was the most effective stimulator of macrophage activation, which occurred earlier and persisted longer than it did with the other agents. Macrophages from animals receiving each of the agents showed enhanced pinocytosis. Measurement of macrophage random migration, chemotaxis, and pinocytosis appeared to provide a rapid and quantitative assessment of several parameters of macrophage function and, when studied with other immunologic parameters, may provide useful tools for the evaluation of potential immunoadjuvants.",

author = "Sher, {Neal A.} and Poplack, {David G.} and {Michael Blaese}, R. and Brown, {Teresa M.} and Chaparas, {Sotiros D.}",

note = "Funding Information: ABBREVIATIONS USED: MER = methanol-extraction residue of BCG; LMS = levamisole; SSV = steroid suspending vehicle; PEC = peritoneal exudate cells; GBBS = Gey's balanced salt solution; cpm = counts per minute. 1 Received August 25, 1976; accepted January 5, 1977. 2 Mycobacterial and Fungal Antigen Branch, Division of Bacterial Products, Bureau of Biologics, U.S. Food and Drug Administration, Bethesda, Md. 20014. 3 Present address: Department of Ophthalmology, University of Minnesota School of Medicine, Mayo Memorial Bldg., Box 449, Minneapolis, Minn. 55455. 4 Pediatric Oncology Branch, Division of Cancer Treatment, National Cancer Institute (NCI), National Institutes of Health, Public Health Service, U. S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. 5 Metabolism Branch, Division of Cancer Biology and Diagnosis, NCI.",

year = "1977",

month = jun,

doi = "10.1093/jnci/58.6.1753",

language = "English (US)",

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journal = "Journal of the National Cancer Institute",

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AU - Sher, Neal A.

AU - Poplack, David G.

AU - Michael Blaese, R.

AU - Brown, Teresa M.

AU - Chaparas, Sotiros D.

N1 - Funding Information: ABBREVIATIONS USED: MER = methanol-extraction residue of BCG; LMS = levamisole; SSV = steroid suspending vehicle; PEC = peritoneal exudate cells; GBBS = Gey's balanced salt solution; cpm = counts per minute. 1 Received August 25, 1976; accepted January 5, 1977. 2 Mycobacterial and Fungal Antigen Branch, Division of Bacterial Products, Bureau of Biologics, U.S. Food and Drug Administration, Bethesda, Md. 20014. 3 Present address: Department of Ophthalmology, University of Minnesota School of Medicine, Mayo Memorial Bldg., Box 449, Minneapolis, Minn. 55455. 4 Pediatric Oncology Branch, Division of Cancer Treatment, National Cancer Institute (NCI), National Institutes of Health, Public Health Service, U. S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. 5 Metabolism Branch, Division of Cancer Biology and Diagnosis, NCI.

PY - 1977/6

Y1 - 1977/6

N2 - Three parameters of macrophage function: Random migration, chemotaxis, and pinocytosis, were studied in the guinea pig after administration of Corynebacterlum parvum, methanol-extraction residue of BCG, and levamisole (LMS), a synthetic anthelmintic. Macrophage migration studies were performed with a modified Boyden chamber. Pinocytosis was assessed by the uptake of colloidal198Au. After ip administration, each of the three immunostimulators induced an increase in macrophage chemotactic responsiveness and, to a lesser extent and duration, in random motility. Kinetic, dose-response, and time course data for the effect of each agent on macrophage movement were explored. LMS was the most effective stimulator of macrophage activation, which occurred earlier and persisted longer than it did with the other agents. Macrophages from animals receiving each of the agents showed enhanced pinocytosis. Measurement of macrophage random migration, chemotaxis, and pinocytosis appeared to provide a rapid and quantitative assessment of several parameters of macrophage function and, when studied with other immunologic parameters, may provide useful tools for the evaluation of potential immunoadjuvants.

AB - Three parameters of macrophage function: Random migration, chemotaxis, and pinocytosis, were studied in the guinea pig after administration of Corynebacterlum parvum, methanol-extraction residue of BCG, and levamisole (LMS), a synthetic anthelmintic. Macrophage migration studies were performed with a modified Boyden chamber. Pinocytosis was assessed by the uptake of colloidal198Au. After ip administration, each of the three immunostimulators induced an increase in macrophage chemotactic responsiveness and, to a lesser extent and duration, in random motility. Kinetic, dose-response, and time course data for the effect of each agent on macrophage movement were explored. LMS was the most effective stimulator of macrophage activation, which occurred earlier and persisted longer than it did with the other agents. Macrophages from animals receiving each of the agents showed enhanced pinocytosis. Measurement of macrophage random migration, chemotaxis, and pinocytosis appeared to provide a rapid and quantitative assessment of several parameters of macrophage function and, when studied with other immunologic parameters, may provide useful tools for the evaluation of potential immunoadjuvants.

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ER -

Effect of corynebacterium parvum, methanol-extraction residue of BCG, and levamisole on macrophage random Migration, Chemotaxis, and Pinocytosis

Abstract

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