Effect of cyclic loading on the nanoscale deformation of hydroxyapatite and collagen fibrils in bovine bone

Anjali Singhal*, Stuart R Stock, Jonathan D. Almer, David C Dunand

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cyclic compressive loading tests were carried out on bovine femoral bones at body temperature (37 °C), with varying mean stresses (-55 to -80 MPa) and loading frequencies (0.5-5 Hz). At various times, the cyclic loading was interrupted to carry out high-energy X-ray scattering measurements of the internal strains developing in the hydroxyapatite (HAP) platelets and the collagen fibrils. The residual strains upon unloading were always tensile in the HAP and compressive in the fibrils, and each increases in magnitude with loading cycles, which can be explained from damage at the HAP-collagen interface and accumulation of plastic deformation within the collagen phase. The samples tested at a higher mean stress and stress amplitude, and at lower loading frequencies exhibit greater plastic deformation and damage accumulation, which is attributed to greater contribution of creep. Synchrotron microcomputed tomography of some of the specimens showed that cracks are produced during cyclic loading and that they mostly occur concentric with Haversian canals.

Original languageEnglish (US)
Pages (from-to)615-626
Number of pages12
JournalBiomechanics and Modeling in Mechanobiology
Volume13
Issue number3
DOIs
StatePublished - Jan 1 2014

Fingerprint

Hydroxyapatite
Cyclic Loading
Collagen
Durapatite
Bone
Bone and Bones
Plastics
Plastic deformation
Plastic Deformation
Haversian System
X-Ray Microtomography
Synchrotrons
Canals
Platelets
Thigh
Damage Accumulation
Body Temperature
Unloading
X ray scattering
Tomography

Keywords

  • Bone
  • Fatigue
  • Interfacial damage
  • Synchrotron
  • X-ray diffraction

ASJC Scopus subject areas

  • Biotechnology
  • Modeling and Simulation
  • Mechanical Engineering

Cite this

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Effect of cyclic loading on the nanoscale deformation of hydroxyapatite and collagen fibrils in bovine bone. / Singhal, Anjali; Stock, Stuart R; Almer, Jonathan D.; Dunand, David C.

In: Biomechanics and Modeling in Mechanobiology, Vol. 13, No. 3, 01.01.2014, p. 615-626.

Research output: Contribution to journalArticle

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AU - Stock, Stuart R

AU - Almer, Jonathan D.

AU - Dunand, David C

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AB - Cyclic compressive loading tests were carried out on bovine femoral bones at body temperature (37 °C), with varying mean stresses (-55 to -80 MPa) and loading frequencies (0.5-5 Hz). At various times, the cyclic loading was interrupted to carry out high-energy X-ray scattering measurements of the internal strains developing in the hydroxyapatite (HAP) platelets and the collagen fibrils. The residual strains upon unloading were always tensile in the HAP and compressive in the fibrils, and each increases in magnitude with loading cycles, which can be explained from damage at the HAP-collagen interface and accumulation of plastic deformation within the collagen phase. The samples tested at a higher mean stress and stress amplitude, and at lower loading frequencies exhibit greater plastic deformation and damage accumulation, which is attributed to greater contribution of creep. Synchrotron microcomputed tomography of some of the specimens showed that cracks are produced during cyclic loading and that they mostly occur concentric with Haversian canals.

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