Effect of hypolipidemic peroxisome proliferators on unscheduled DNA synthesis in cultured hepatocytes and on mutagenesis in Salmonella

Howard P. Glauert; Janardan K. Reddy; Wendy S. Kennan; Gerald L. Sattler; V. Subba Rao; Henry C. Pitot

doi:10.1016/0304-3835(84)90130-7

Effect of hypolipidemic peroxisome proliferators on unscheduled DNA synthesis in cultured hepatocytes and on mutagenesis in Salmonella

Howard P. Glauert^*, Janardan K. Reddy, Wendy S. Kennan, Gerald L. Sattler, V. Subba Rao, Henry C. Pitot

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

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Abstract

The peroxisome proliferators Wy-14,643, BR-931, nafenopin and ciprofibrate were tested in the primary hepatocyte culture-unscheduled DNA synthesis assay and in the Ames Salmonella microsome mutagenicity assay. The amount of unscheduled DNA synthesis (UDS) in hepatocytes was determined by quantifying the amount of [³H] thymidine incorporated into DNA in the presence of hydroxyurea after isolation of nuclei from hepatocytes treated with the test agent. Wy-14,643 and BR-931 induced unscheduled DNA synthesis in rat hepatocytes, whereas nafenopin and ciprofibrate had no effect. All of the peroxisome proliferators were negative in the Ames Salmonella assay.

Original language	English (US)
Pages (from-to)	147-156
Number of pages	10
Journal	Cancer Letters
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(84)90130-7
State	Published - Sep 1984

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3835(84)90130-7

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@article{eea7ba7a80cd46ba9754e754bd15a207,

title = "Effect of hypolipidemic peroxisome proliferators on unscheduled DNA synthesis in cultured hepatocytes and on mutagenesis in Salmonella",

abstract = "The peroxisome proliferators Wy-14,643, BR-931, nafenopin and ciprofibrate were tested in the primary hepatocyte culture-unscheduled DNA synthesis assay and in the Ames Salmonella microsome mutagenicity assay. The amount of unscheduled DNA synthesis (UDS) in hepatocytes was determined by quantifying the amount of [3H] thymidine incorporated into DNA in the presence of hydroxyurea after isolation of nuclei from hepatocytes treated with the test agent. Wy-14,643 and BR-931 induced unscheduled DNA synthesis in rat hepatocytes, whereas nafenopin and ciprofibrate had no effect. All of the peroxisome proliferators were negative in the Ames Salmonella assay.",

author = "Glauert, {Howard P.} and Reddy, {Janardan K.} and Kennan, {Wendy S.} and Sattler, {Gerald L.} and {Subba Rao}, V. and Pitot, {Henry C.}",

note = "Funding Information: This work was supported by DHHS PHS National Research Service Award 5 T32 ES07015 from the National Institute of Environmental Health Sciences, Grants CA-07175 and CA-22484 from the National Cancer Institute, and Grant GM-23750 from the National Institutes of Health.",

year = "1984",

month = sep,

doi = "10.1016/0304-3835(84)90130-7",

language = "English (US)",

volume = "24",

pages = "147--156",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

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TY - JOUR

T1 - Effect of hypolipidemic peroxisome proliferators on unscheduled DNA synthesis in cultured hepatocytes and on mutagenesis in Salmonella

AU - Glauert, Howard P.

AU - Reddy, Janardan K.

AU - Kennan, Wendy S.

AU - Sattler, Gerald L.

AU - Subba Rao, V.

AU - Pitot, Henry C.

N1 - Funding Information: This work was supported by DHHS PHS National Research Service Award 5 T32 ES07015 from the National Institute of Environmental Health Sciences, Grants CA-07175 and CA-22484 from the National Cancer Institute, and Grant GM-23750 from the National Institutes of Health.

PY - 1984/9

Y1 - 1984/9

N2 - The peroxisome proliferators Wy-14,643, BR-931, nafenopin and ciprofibrate were tested in the primary hepatocyte culture-unscheduled DNA synthesis assay and in the Ames Salmonella microsome mutagenicity assay. The amount of unscheduled DNA synthesis (UDS) in hepatocytes was determined by quantifying the amount of [3H] thymidine incorporated into DNA in the presence of hydroxyurea after isolation of nuclei from hepatocytes treated with the test agent. Wy-14,643 and BR-931 induced unscheduled DNA synthesis in rat hepatocytes, whereas nafenopin and ciprofibrate had no effect. All of the peroxisome proliferators were negative in the Ames Salmonella assay.

AB - The peroxisome proliferators Wy-14,643, BR-931, nafenopin and ciprofibrate were tested in the primary hepatocyte culture-unscheduled DNA synthesis assay and in the Ames Salmonella microsome mutagenicity assay. The amount of unscheduled DNA synthesis (UDS) in hepatocytes was determined by quantifying the amount of [3H] thymidine incorporated into DNA in the presence of hydroxyurea after isolation of nuclei from hepatocytes treated with the test agent. Wy-14,643 and BR-931 induced unscheduled DNA synthesis in rat hepatocytes, whereas nafenopin and ciprofibrate had no effect. All of the peroxisome proliferators were negative in the Ames Salmonella assay.

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U2 - 10.1016/0304-3835(84)90130-7

DO - 10.1016/0304-3835(84)90130-7

M3 - Article

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AN - SCOPUS:0021174837

SN - 0304-3835

VL - 24

SP - 147

EP - 156

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Effect of hypolipidemic peroxisome proliferators on unscheduled DNA synthesis in cultured hepatocytes and on mutagenesis in Salmonella

Abstract

ASJC Scopus subject areas

UN SDGs

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