TY - JOUR
T1 - Effect of Inosine on Contractile Force and High-Energy Phosphates in Ischemic Hearts
AU - Thomas, J. X.
AU - Jones, C. E.
N1 - Funding Information:
This work was supported in part by NIH Program-Project HL 08682, Grant HL 20286, and Contract 1-HV-22948. 2
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1979/9
Y1 - 1979/9
N2 - The effects of intravenously infused inosine on myocardial contractile force (MCF) and high-energy phosphate content were studied in 17 open-chest, pentobarbital-anesthetized mongrel dogs following occlusion of small branches of the left anterior descending and circumflex coronary arteries. In 7 dogs, MCF was measured using strain gauge arches sutured in the center of the ischemic area (IMCF), in the periphery of the ischemic area (PIMCF), and in a nonischemic area (NIMCF). After the arteries were ligated and IMCF had stabilized, an infusion of either 50 ml of 50 mM inosine or saline was begun at a rate of 10 ml/min. Inosine infusion produced increases in IMCF, PIMCF, and NIMCF of 32, 41, and 42% of preinfusion levels, respectively. In an additional 10 dogs, adenosine triphosphate (ATP) and creatine phosphate (CP) content of the ischemic myocardium was determined at intervals prior to and following coronary arterial occlusion, during which time either inosine (n = 5) or normal saline (n = 5) was infused. The degradation of ATP or CP was not altered with inosine infusion as compared to the saline-infused group. It has been concluded from the above data that inosine significantly increases MCF in ischemic hearts and that this augmentation in MCF is not associated with alterations in levels of ATP or CP.
AB - The effects of intravenously infused inosine on myocardial contractile force (MCF) and high-energy phosphate content were studied in 17 open-chest, pentobarbital-anesthetized mongrel dogs following occlusion of small branches of the left anterior descending and circumflex coronary arteries. In 7 dogs, MCF was measured using strain gauge arches sutured in the center of the ischemic area (IMCF), in the periphery of the ischemic area (PIMCF), and in a nonischemic area (NIMCF). After the arteries were ligated and IMCF had stabilized, an infusion of either 50 ml of 50 mM inosine or saline was begun at a rate of 10 ml/min. Inosine infusion produced increases in IMCF, PIMCF, and NIMCF of 32, 41, and 42% of preinfusion levels, respectively. In an additional 10 dogs, adenosine triphosphate (ATP) and creatine phosphate (CP) content of the ischemic myocardium was determined at intervals prior to and following coronary arterial occlusion, during which time either inosine (n = 5) or normal saline (n = 5) was infused. The degradation of ATP or CP was not altered with inosine infusion as compared to the saline-infused group. It has been concluded from the above data that inosine significantly increases MCF in ischemic hearts and that this augmentation in MCF is not associated with alterations in levels of ATP or CP.
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U2 - 10.3181/00379727-161-40575
DO - 10.3181/00379727-161-40575
M3 - Article
C2 - 482277
AN - SCOPUS:0018692959
SN - 0037-9727
VL - 161
SP - 468
EP - 472
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 4
ER -