The intracellular free calcium ion concentration ([Ca2+]i) of the neuroblastoma × glioma hybrid cell line, NG108-15, was measured using the 19F-nuclear magnetic resonance divalent cation indicator, 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N′,N′-tetra-acetic acid (5F-BAPTA). The basal [Ca2+]i was measured to be 106 ± 14 nM. Treatment with 5 μM lead (Pb) for 2 h produced a 2-fold increase in [Ca2+]i to 200 ± 24 nM and a measurable intracellular free Pb2+ concentration ([Pb2+]i) of 30 ± 10 pM. Intracellular free Zn2+ concentrations ([Zn2+]i) were also observed in the presence of Pb. This represents the first direct demonstration that Pb elevates the [Ca2+]i in neurons, thus providing evidence for a role of [Ca2+]i in mediating the neurotoxicity of Pb.
- Nuclear magnetic resonance spectroscopy, F
ASJC Scopus subject areas
- Molecular Biology
- Clinical Neurology
- Developmental Biology