Effect of lead on intracellular free calcium ion concentration in a presynaptic neuronal model: 19F-NMR study of NG108-15 cells

Francis A X Schanne, Joseph R. Moskal, R. K. Gupta

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The intracellular free calcium ion concentration ([Ca2+]i) of the neuroblastoma × glioma hybrid cell line, NG108-15, was measured using the 19F-nuclear magnetic resonance divalent cation indicator, 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N′,N′-tetra-acetic acid (5F-BAPTA). The basal [Ca2+]i was measured to be 106 ± 14 nM. Treatment with 5 μM lead (Pb) for 2 h produced a 2-fold increase in [Ca2+]i to 200 ± 24 nM and a measurable intracellular free Pb2+ concentration ([Pb2+]i) of 30 ± 10 pM. Intracellular free Zn2+ concentrations ([Zn2+]i) were also observed in the presence of Pb. This represents the first direct demonstration that Pb elevates the [Ca2+]i in neurons, thus providing evidence for a role of [Ca2+]i in mediating the neurotoxicity of Pb.

Original languageEnglish (US)
Pages (from-to)308-311
Number of pages4
JournalBrain research
Volume503
Issue number2
DOIs
StatePublished - Dec 4 1989

Keywords

  • Calcium
  • Lead
  • NG108-15
  • Neuron
  • Nuclear magnetic resonance spectroscopy, F

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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