Effect of liposome-encapsulated hemoglobin on the development of endotoxin-induced shock in the rat

M. Whiteford, A. Spirig, A. Rudolph, L. Neville, F. Abdullah, G. Feuerstein, Reuven Rabinovici*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Liposome-encapsulated hemoglobin (LEH) is an experimental oxygen-carrying resuscitation fluid. Because LEH is cleared from the circulation primarily by the reticuloendothelial system, its effect on the development of sepsis remains a major concern. Thus, the present study aimed to evaluate whether LEH modifies consequences of endotoxemia in the conscious normovolemic rat. LEH infusion at 10% of estimated blood volume (n = 10) did not affect mortality (30%, p < .05) and serum tumor necrosis factor-α levels (6204 ± 414, p < .05) induced by 3.6 mg/kg Escherichia coli endotoxin administered (intravenous bolus) 22 h later. In contrast, when a shorter LEH-endotoxin time interval (<12 h, n = 10) or a higher dose of endotoxin (14.4 mg/kg, n = 20) was tested, LEH enhanced endotoxin-induced mortality (90% and 100%, respectively, p < .05) and broadened serum tumor necrosis factor-α response without modifying its peak levels. LEH (n = 20) did not exacerbate the endotoxin-induced tachycardia, leukopenia, and thrombocytopenia. Therefore, in this model, the effect of LEH on endotoxin-induced responses was dependent on the time interval between LEH and endotoxin administration as well as the endotoxin dose. The clinical relevance of these results should be further investigated.

Original languageEnglish (US)
Pages (from-to)428-433
Number of pages6
JournalShock
Volume9
Issue number6
DOIs
StatePublished - Jan 1 1998

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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    Whiteford, M., Spirig, A., Rudolph, A., Neville, L., Abdullah, F., Feuerstein, G., & Rabinovici, R. (1998). Effect of liposome-encapsulated hemoglobin on the development of endotoxin-induced shock in the rat. Shock, 9(6), 428-433. https://doi.org/10.1097/00024382-199806000-00007