TY - JOUR
T1 - Effect of MAP 1, MAP 2, and tau-proteins on structural parameters of tubulin assemblies.
AU - Böhm, K. J.
AU - Vater, W.
AU - Steinmetzer, P.
AU - Kusnetsov, S. A.
AU - Rodionov, V. I.
AU - Gelfand, V. I.
AU - Unger, E.
PY - 1990
Y1 - 1990
N2 - When temperature-dependent recycling procedures for purification were used, tubulin is usually accompanied by a mixture of microtubule-associated proteins (MAPs) primarily comprising MAP 1, MAP 2, and the tau-proteins. Formerly we reported that microtubules formed from tubulin in the presence of these MAPs have more protofilaments than those formed without MAPs. Furthermore, these MAPs suppress the formation of aberrant assemblies (Böhm et al., BBA 800, 119, 1984). Now, we report that each single MAP fraction is able to influence the protofilament number of microtubules and the ratio between aberrant assemblies and microtubules in the same manner as the MAP mixture. This implies that all the MAPs investigated play a certain role in lateral association of tubulin dimers.
AB - When temperature-dependent recycling procedures for purification were used, tubulin is usually accompanied by a mixture of microtubule-associated proteins (MAPs) primarily comprising MAP 1, MAP 2, and the tau-proteins. Formerly we reported that microtubules formed from tubulin in the presence of these MAPs have more protofilaments than those formed without MAPs. Furthermore, these MAPs suppress the formation of aberrant assemblies (Böhm et al., BBA 800, 119, 1984). Now, we report that each single MAP fraction is able to influence the protofilament number of microtubules and the ratio between aberrant assemblies and microtubules in the same manner as the MAP mixture. This implies that all the MAPs investigated play a certain role in lateral association of tubulin dimers.
UR - http://www.scopus.com/inward/record.url?scp=0025619038&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025619038&partnerID=8YFLogxK
M3 - Article
C2 - 2127856
AN - SCOPUS:0025619038
VL - 39
SP - 357
EP - 364
JO - Acta histochemica. Supplementband
JF - Acta histochemica. Supplementband
SN - 0567-7556
ER -