Effect of microRNA-10a on migration and invasion of gastric cancer cell line BGC823

Objective: To investigate the effect of human microRNA-10a(miR-10a) on the migration and invasion of gastric cancer cell line BGC823. Methods: The Transwell system was used to select highly invasive sub-cell lines from gastric cancer cell line BGC823. Using miRNA microchip, we compared the miRNA expression in paired cell lines with high and low invasive potentials. MiR-10a was relatively overexpressed in the highly invasive cell lines when compared with its counterpart. The mature type human miR-10a was synthesized chemically. The synthesized miR-10a (25, 50, 100, and 150 nmol/L) was transfected into BGC823 cells via lipofectamin 2000. Cells were also transfected with empty vectors and unrelated fragment to serve as controls. The expression of mature type miR-10a was detected by real-time PCR. Cell counting kit-8 was used to study the effect of miR-10a on the proliferation of BGC823 cells. Flow cytometry was performed to detect the effect of miR-10a on the apoptosis of BGC823 cells. The migration and invasion of BGC823 cells were investigated by Transwell assay. Results: Real-time PCR showed that cells transfected with mature type miR-10a had significantly higher expression of miR-10a, with the optimal concentration of miR-10a being 100 nmol/L; the associated miR-10a expression was 2.06 folds that of unrelated group. miR-10a (100 nmoL/L) had no obvious influence on the proliferation and apoptosis of BGC823 cells; however, it promoted the migration and invasion of BGC823 cells, with the promoting rates being (88.34 ± 0.61)% and (56.02 ± 3.13)%, respectively. Conclusion: Synthesized mature type human miR-10a can effectively enhance miR-10a expression and promote the migration and invasion of the BGC823 cells.