Effect of phorbol esters on the susceptibility of a glioma cell line to lymphokine-activated killer cell activity

A. Maleci; R. L. Alterman; D. Sundstrom; P. L. Kornblith; J. R. Moskal

doi:10.3171/jns.1990.73.1.0091

Effect of phorbol esters on the susceptibility of a glioma cell line to lymphokine-activated killer cell activity

A. Maleci, R. L. Alterman, D. Sundstrom, P. L. Kornblith, J. R. Moskal^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The mechanisms by which lymphokine-activated killer (LAK) cells exert their cytotoxic effects are not well understood. This study demonstrates that phorbol ester pretreatment of a LAK cell-sensitive glioma cell line (SNB-19) induced a significant decrease in the susceptibility of cells to LAK cell-mediated lysis. This effect was produced by low concentrations of the tumor-promoting phorbol ester, phorbol-12,13-myristate acetate (PMA), and was reversible. Protein kinase C (PKC) inhibitors failed to block this phenomenon. No apparent alteration in the ability of LAK cells to bind to their targets was observed. Thus, PMA may have exerted its effects by a mechanism that does not require PKC, or these glioma cells may possess an isozyme of PKC which is insensitive to the inhibitors used in these studies.

Original language	English (US)
Pages (from-to)	91-97
Number of pages	7
Journal	Journal of neurosurgery
Volume	73
Issue number	1
DOIs	https://doi.org/10.3171/jns.1990.73.1.0091
State	Published - Jan 1 1990

Keywords

Cytotoxicity
Glioma
Interleukin-2
Lymphokine-activated killer cell
Phorbol ester
Protein kinase C

ASJC Scopus subject areas

Surgery
Clinical Neurology

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title = "Effect of phorbol esters on the susceptibility of a glioma cell line to lymphokine-activated killer cell activity",

abstract = "The mechanisms by which lymphokine-activated killer (LAK) cells exert their cytotoxic effects are not well understood. This study demonstrates that phorbol ester pretreatment of a LAK cell-sensitive glioma cell line (SNB-19) induced a significant decrease in the susceptibility of cells to LAK cell-mediated lysis. This effect was produced by low concentrations of the tumor-promoting phorbol ester, phorbol-12,13-myristate acetate (PMA), and was reversible. Protein kinase C (PKC) inhibitors failed to block this phenomenon. No apparent alteration in the ability of LAK cells to bind to their targets was observed. Thus, PMA may have exerted its effects by a mechanism that does not require PKC, or these glioma cells may possess an isozyme of PKC which is insensitive to the inhibitors used in these studies.",

keywords = "Cytotoxicity, Glioma, Interleukin-2, Lymphokine-activated killer cell, Phorbol ester, Protein kinase C",

author = "A. Maleci and Alterman, {R. L.} and D. Sundstrom and Kornblith, {P. L.} and Moskal, {J. R.}",

year = "1990",

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AU - Maleci, A.

AU - Alterman, R. L.

AU - Sundstrom, D.

AU - Kornblith, P. L.

AU - Moskal, J. R.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The mechanisms by which lymphokine-activated killer (LAK) cells exert their cytotoxic effects are not well understood. This study demonstrates that phorbol ester pretreatment of a LAK cell-sensitive glioma cell line (SNB-19) induced a significant decrease in the susceptibility of cells to LAK cell-mediated lysis. This effect was produced by low concentrations of the tumor-promoting phorbol ester, phorbol-12,13-myristate acetate (PMA), and was reversible. Protein kinase C (PKC) inhibitors failed to block this phenomenon. No apparent alteration in the ability of LAK cells to bind to their targets was observed. Thus, PMA may have exerted its effects by a mechanism that does not require PKC, or these glioma cells may possess an isozyme of PKC which is insensitive to the inhibitors used in these studies.

AB - The mechanisms by which lymphokine-activated killer (LAK) cells exert their cytotoxic effects are not well understood. This study demonstrates that phorbol ester pretreatment of a LAK cell-sensitive glioma cell line (SNB-19) induced a significant decrease in the susceptibility of cells to LAK cell-mediated lysis. This effect was produced by low concentrations of the tumor-promoting phorbol ester, phorbol-12,13-myristate acetate (PMA), and was reversible. Protein kinase C (PKC) inhibitors failed to block this phenomenon. No apparent alteration in the ability of LAK cells to bind to their targets was observed. Thus, PMA may have exerted its effects by a mechanism that does not require PKC, or these glioma cells may possess an isozyme of PKC which is insensitive to the inhibitors used in these studies.

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KW - Glioma

KW - Interleukin-2

KW - Lymphokine-activated killer cell

KW - Phorbol ester

KW - Protein kinase C

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