Effect of phorbol esters on the susceptibility of a glioma cell line to lymphokine-activated killer cell activity

A. Maleci, R. L. Alterman, D. Sundstrom, P. L. Kornblith, J. R. Moskal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanisms by which lymphokine-activated killer (LAK) cells exert their cytotoxic effects are not well understood. This study demonstrates that phorbol ester pretreatment of a LAK cell-sensitive glioma cell line (SNB-19) induced a significant decrease in the susceptibility of cells to LAK cell-mediated lysis. This effect was produced by low concentrations of the tumor-promoting phorbol ester, phorbol-12,13-myristate acetate (PMA), and was reversible. Protein kinase C (PKC) inhibitors failed to block this phenomenon. No apparent alteration in the ability of LAK cells to bind to their targets was observed. Thus, PMA may have exerted its effects by a mechanism that does not require PKC, or these glioma cells may possess an isozyme of PKC which is insensitive to the inhibitors used in these studies.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalJournal of neurosurgery
Volume73
Issue number1
DOIs
StatePublished - Jan 1 1990

Keywords

  • Cytotoxicity
  • Glioma
  • Interleukin-2
  • Lymphokine-activated killer cell
  • Phorbol ester
  • Protein kinase C

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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