Effect of propionic and methylmalonic acids on the high molecular weight neurofilament subunit (NF-H) in rat cerebral cortex

L. Vivian, F. Dall Bello Pessutto, L. M. Vieira De Almeida, S. De Oliveira Loureiro, P. De Lima Pelaez, C. Funchal, M. Wajner, Regina Pessoa-Pureur*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Propionic and methylmalonic acidemias are inherited neurometabolic disorders biochemically characterized by tissue accumulation of propionic (PA) and methylmalonic (MMA) acids, respectively. Neurofilaments (NF) are important cytoskeletal proteins and phosphorylation/dephosphorylation of NF is important to stabilize the cytoskeleton. We investigated the effects of PA and MMA on the high molecular weight neurofilament subunit associated with the cytoskeletal fraction of rat cerebral cortex along development. Cortical slices from 9- to 60-dayold rats were incubated with 2.5 mM PA or MMA. The cytoskeletal fraction was extracted and the immunoreactivity for phosphorylated or total NF-H was analyzed by immunoblotting using specific antibodies. Results showed that treatment of tissue slices with the acids induced an increased Triton-insoluble phosphorylated NF-H immunoreactivity in up to 17-day-old rats. Furthermore, treatments significantly increased the total amount of NF-H in 12-day-old rats. These findings indicate that PA and MMA alter the dynamic regulation of NF-H assembly in the cytoskeletal fraction.

Original languageEnglish (US)
Pages (from-to)1691-1697
Number of pages7
JournalNeurochemical Research
Volume27
Issue number12
DOIs
StatePublished - Dec 1 2002

Keywords

  • Cytoskeleton
  • Development
  • Methylmalonic acid
  • Neurofilament
  • Phosphorylation
  • Propionic acid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Vivian, L., Dall Bello Pessutto, F., Vieira De Almeida, L. M., De Oliveira Loureiro, S., De Lima Pelaez, P., Funchal, C., Wajner, M., & Pessoa-Pureur, R. (2002). Effect of propionic and methylmalonic acids on the high molecular weight neurofilament subunit (NF-H) in rat cerebral cortex. Neurochemical Research, 27(12), 1691-1697. https://doi.org/10.1023/A:1021699314007