Effect of protein concentration on kinetic constants (Kd and Bmax) of 3H-Imipramine binding in blood platelets

Ramesh C. Arora; Vala Wunnicke; Herbert Y. Meltzer

doi:10.1016/0006-3223(85)90146-5

Effect of protein concentration on kinetic constants (K_d and B_max) of ³H-Imipramine binding in blood platelets

Ramesh C. Arora^*, Vala Wunnicke, Herbert Y. Meltzer

^*Corresponding author for this work

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

³H-imipramine binding was studied in the blood platelets of 80 normal controls and 50 depressed (psychotic and nonpsychotic) patients. Protein concentration in the incubation mixture, and K_d and B_max values were correlated in normal controls and depressed patients. A small but significant correlation between protein in the incubation mixture and B_max was observed in normal controls and depressed patients. There was also a significant correlation between protein and K_d of imipramine binding in the blood platelets of unipolar psychotic depressed patients. However, analysis of covariance to remove the effect of protein on K_d and B_max did not change the basic finding of decreased B_max in the blood platelets of psychotic depressed patients.

Original language	English (US)
Pages (from-to)	116-119
Number of pages	4
Journal	Biological psychiatry
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/0006-3223(85)90146-5
State	Published - Jan 1985

ASJC Scopus subject areas

Biological Psychiatry

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title = "Effect of protein concentration on kinetic constants (Kd and Bmax) of 3H-Imipramine binding in blood platelets",

abstract = "3H-imipramine binding was studied in the blood platelets of 80 normal controls and 50 depressed (psychotic and nonpsychotic) patients. Protein concentration in the incubation mixture, and Kd and Bmax values were correlated in normal controls and depressed patients. A small but significant correlation between protein in the incubation mixture and Bmax was observed in normal controls and depressed patients. There was also a significant correlation between protein and Kd of imipramine binding in the blood platelets of unipolar psychotic depressed patients. However, analysis of covariance to remove the effect of protein on Kd and Bmax did not change the basic finding of decreased Bmax in the blood platelets of psychotic depressed patients.",

author = "Arora, {Ramesh C.} and Vala Wunnicke and Meltzer, {Herbert Y.}",

note = "Funding Information: Supported in part by USPHS MH 30,059 and 30,938 and Research Career ScientistA ward MH 47,808 (Dr. Meltzer) and by the Illinois Departmento f Mental Health.",

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AU - Arora, Ramesh C.

AU - Wunnicke, Vala

AU - Meltzer, Herbert Y.

N1 - Funding Information: Supported in part by USPHS MH 30,059 and 30,938 and Research Career ScientistA ward MH 47,808 (Dr. Meltzer) and by the Illinois Departmento f Mental Health.

PY - 1985/1

Y1 - 1985/1

N2 - 3H-imipramine binding was studied in the blood platelets of 80 normal controls and 50 depressed (psychotic and nonpsychotic) patients. Protein concentration in the incubation mixture, and Kd and Bmax values were correlated in normal controls and depressed patients. A small but significant correlation between protein in the incubation mixture and Bmax was observed in normal controls and depressed patients. There was also a significant correlation between protein and Kd of imipramine binding in the blood platelets of unipolar psychotic depressed patients. However, analysis of covariance to remove the effect of protein on Kd and Bmax did not change the basic finding of decreased Bmax in the blood platelets of psychotic depressed patients.

AB - 3H-imipramine binding was studied in the blood platelets of 80 normal controls and 50 depressed (psychotic and nonpsychotic) patients. Protein concentration in the incubation mixture, and Kd and Bmax values were correlated in normal controls and depressed patients. A small but significant correlation between protein in the incubation mixture and Bmax was observed in normal controls and depressed patients. There was also a significant correlation between protein and Kd of imipramine binding in the blood platelets of unipolar psychotic depressed patients. However, analysis of covariance to remove the effect of protein on Kd and Bmax did not change the basic finding of decreased Bmax in the blood platelets of psychotic depressed patients.

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Effect of protein concentration on kinetic constants (K_d and B_max) of ³H-Imipramine binding in blood platelets

Abstract

ASJC Scopus subject areas

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