Abstract
The hemostatic effect of recombinant (r)-aprotinin was studied in 41 patients undergoing cardiopulmonary bypass surgery. Flow cytometry was used to measure the expression of glycoprotein 1b (GP1b) and α-granule membrane protein 140 (GMP-140) on platelets. Additional parameters studied were β-thromboglobulin (β-TG), fibrinogen, fibrinopeptide A, plasminogen, platelet count, and the amount of postoperative chest tube drainage. Chest tube drainage was significantly less in the r-aprotinin-treated patients (p < 0.001). The percentage of platelets expressing GMP-140 increased to 9.7% in r-apro- tinin patients and to 12.1 % in controls during the surgery. The difference between both groups was not significant. Both values returned to baseline the next day. GP1b expression decreased in both groups by 33-38% during the surgery and returned to baseline the next day. GP1b expression and the plasma concentrations of fibrinogen, fibrinopeptide A, β-TG, and plasminogen were not different in r-aprotinin and control patients. We conclude that r-aprotinin reduces blood loss during cardiopulmonary bypass surgery, but the primary mechanism is likely not through a protective effect on platelets.
Original language | English (US) |
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Pages (from-to) | 293-300 |
Number of pages | 8 |
Journal | Pathophysiology of Haemostasis and Thrombosis |
Volume | 23 |
Issue number | 6 |
DOIs | |
State | Published - 1993 |
Keywords
- Aprotinin
- Cardiopulmonary bypass
- Flow cytometry
- Granule membrane
- Platelet function
- Protein 140 Glycoprotein 1b
- Surgery
ASJC Scopus subject areas
- Physiology (medical)
- Hematology