Effect of recombinant human c-kit receptor ligand on mediator release from human skin mast cells

Michele Columbo; Edward M. Horowitz; Luis M. Botana; Donald W. Macglasha; Bruce S. Bochner; Steven Gillis; Krisztina M. Zsebo; Stephen J. Galli; Lawrence M. Lichtenstein

doi:10.1159/000236274

Effect of recombinant human c-kit receptor ligand on mediator release from human skin mast cells

Michele Columbo^*, Edward M. Horowitz, Luis M. Botana, Donald W. Macglasha, Bruce S. Bochner, Steven Gillis, Krisztina M. Zsebo, Stephen J. Galli, Lawrence M. Lichtenstein

^*Corresponding author for this work

Medicine, Allergy Division

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We studied the activity of recombinant human stem cell factor (rhSCF) on the release of mediators from human skin mast cells. High concentrations of rhSCF (1 ng/ml-1 µg/ml) induced a rapid and sustained rise in intracellular Ca²⁺ levels that was accompanied by release of histamine and prostaglandin D: (PGD:). A brief incubation (10 min) with lower concentrations of rhSCF (0.1 pg/ml-1 ng/ml) enhanced anti-IgE-induced histamine release, but not the release of PGD₂. Since some of the effects of rhSCF on mast cell mediator release in vitro occur at concentrations of cytokine similar to those found in the scrum of normal subjects, it is possible that SCF contributes to the modulation of mast cell function under physiological conditions.

Original language	English (US)
Pages (from-to)	323-325
Number of pages	3
Journal	International archives of allergy and immunology
Volume	99
Issue number	2-4
DOIs	https://doi.org/10.1159/000236274
State	Published - 1992

Keywords

Anti-IgE
Histamine
Mast cells
Prostaglandin D<inf>2</inf>

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1159/000236274

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title = "Effect of recombinant human c-kit receptor ligand on mediator release from human skin mast cells",

abstract = "We studied the activity of recombinant human stem cell factor (rhSCF) on the release of mediators from human skin mast cells. High concentrations of rhSCF (1 ng/ml-1 µg/ml) induced a rapid and sustained rise in intracellular Ca2+ levels that was accompanied by release of histamine and prostaglandin D: (PGD:). A brief incubation (10 min) with lower concentrations of rhSCF (0.1 pg/ml-1 ng/ml) enhanced anti-IgE-induced histamine release, but not the release of PGD2. Since some of the effects of rhSCF on mast cell mediator release in vitro occur at concentrations of cytokine similar to those found in the scrum of normal subjects, it is possible that SCF contributes to the modulation of mast cell function under physiological conditions.",

keywords = "Anti-IgE, Histamine, Mast cells, Prostaglandin D<inf>2</inf>",

author = "Michele Columbo and Horowitz, {Edward M.} and Botana, {Luis M.} and Macglasha, {Donald W.} and Bochner, {Bruce S.} and Steven Gillis and Zsebo, {Krisztina M.} and Galli, {Stephen J.} and Lichtenstein, {Lawrence M.}",

year = "1992",

doi = "10.1159/000236274",

language = "English (US)",

volume = "99",

pages = "323--325",

journal = "International archives of allergy and immunology",

issn = "1018-2438",

publisher = "S. Karger AG",

number = "2-4",

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T1 - Effect of recombinant human c-kit receptor ligand on mediator release from human skin mast cells

AU - Columbo, Michele

AU - Horowitz, Edward M.

AU - Botana, Luis M.

AU - Macglasha, Donald W.

AU - Bochner, Bruce S.

AU - Gillis, Steven

AU - Zsebo, Krisztina M.

AU - Galli, Stephen J.

AU - Lichtenstein, Lawrence M.

PY - 1992

Y1 - 1992

N2 - We studied the activity of recombinant human stem cell factor (rhSCF) on the release of mediators from human skin mast cells. High concentrations of rhSCF (1 ng/ml-1 µg/ml) induced a rapid and sustained rise in intracellular Ca2+ levels that was accompanied by release of histamine and prostaglandin D: (PGD:). A brief incubation (10 min) with lower concentrations of rhSCF (0.1 pg/ml-1 ng/ml) enhanced anti-IgE-induced histamine release, but not the release of PGD2. Since some of the effects of rhSCF on mast cell mediator release in vitro occur at concentrations of cytokine similar to those found in the scrum of normal subjects, it is possible that SCF contributes to the modulation of mast cell function under physiological conditions.

AB - We studied the activity of recombinant human stem cell factor (rhSCF) on the release of mediators from human skin mast cells. High concentrations of rhSCF (1 ng/ml-1 µg/ml) induced a rapid and sustained rise in intracellular Ca2+ levels that was accompanied by release of histamine and prostaglandin D: (PGD:). A brief incubation (10 min) with lower concentrations of rhSCF (0.1 pg/ml-1 ng/ml) enhanced anti-IgE-induced histamine release, but not the release of PGD2. Since some of the effects of rhSCF on mast cell mediator release in vitro occur at concentrations of cytokine similar to those found in the scrum of normal subjects, it is possible that SCF contributes to the modulation of mast cell function under physiological conditions.

KW - Anti-IgE

KW - Histamine

KW - Mast cells

KW - Prostaglandin D<inf>2</inf>

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M3 - Article

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JO - International archives of allergy and immunology

JF - International archives of allergy and immunology

IS - 2-4

ER -

Effect of recombinant human c-kit receptor ligand on mediator release from human skin mast cells

Abstract

Keywords

ASJC Scopus subject areas

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