Effect of streptokinase on fibrinogen and related proteins in plasma

L. J. Bowie; R. Kinney; M. Vye; B. Eckert; H. C. Kwaan

doi:10.1093/clinchem/32.1.67

Effect of streptokinase on fibrinogen and related proteins in plasma

L. J. Bowie, R. Kinney, M. Vye, B. Eckert, H. C. Kwaan

Medicine, Hematology Oncology Division

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Abstract

We investigated the effect of streptokinase on determination of fibrinogen and related clottable proteins in an effort to assess the relative reliability of these determinations in monitoring patients being treated with streptokinase. Citrated plasma was incubated with and without streptokinase, then assayed for fibrinogen (I), fibrin(ogen) degradation products (II), total clottable protein (III), and plasminogen (IV). Values for I decreased rapidly. Values for III and IV generally paralleled those for I, values for III lacked adequate sensitivity. II, low initially, increased to 80 mg/L in 1-2 h. Soybean trypsin inhibitor effectively stabilized all these constituents (except IV) in streptokinase-treated plasma, and we recommend that this (or some other such) inhibitor of fibrinolysis be used routinely when specimens are collected for these determinations. Data are presented that suggest that transient polymers are formed, the clinical significance of which is not known.

Original language	English (US)
Pages (from-to)	67-70
Number of pages	4
Journal	Clinical chemistry
Volume	32
Issue number	1
DOIs	https://doi.org/10.1093/clinchem/32.1.67
State	Published - 1986

ASJC Scopus subject areas

Biochemistry, medical
Clinical Biochemistry

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title = "Effect of streptokinase on fibrinogen and related proteins in plasma",

abstract = "We investigated the effect of streptokinase on determination of fibrinogen and related clottable proteins in an effort to assess the relative reliability of these determinations in monitoring patients being treated with streptokinase. Citrated plasma was incubated with and without streptokinase, then assayed for fibrinogen (I), fibrin(ogen) degradation products (II), total clottable protein (III), and plasminogen (IV). Values for I decreased rapidly. Values for III and IV generally paralleled those for I, values for III lacked adequate sensitivity. II, low initially, increased to 80 mg/L in 1-2 h. Soybean trypsin inhibitor effectively stabilized all these constituents (except IV) in streptokinase-treated plasma, and we recommend that this (or some other such) inhibitor of fibrinolysis be used routinely when specimens are collected for these determinations. Data are presented that suggest that transient polymers are formed, the clinical significance of which is not known.",

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T1 - Effect of streptokinase on fibrinogen and related proteins in plasma

AU - Bowie, L. J.

AU - Kinney, R.

AU - Vye, M.

AU - Eckert, B.

AU - Kwaan, H. C.

PY - 1986

Y1 - 1986

N2 - We investigated the effect of streptokinase on determination of fibrinogen and related clottable proteins in an effort to assess the relative reliability of these determinations in monitoring patients being treated with streptokinase. Citrated plasma was incubated with and without streptokinase, then assayed for fibrinogen (I), fibrin(ogen) degradation products (II), total clottable protein (III), and plasminogen (IV). Values for I decreased rapidly. Values for III and IV generally paralleled those for I, values for III lacked adequate sensitivity. II, low initially, increased to 80 mg/L in 1-2 h. Soybean trypsin inhibitor effectively stabilized all these constituents (except IV) in streptokinase-treated plasma, and we recommend that this (or some other such) inhibitor of fibrinolysis be used routinely when specimens are collected for these determinations. Data are presented that suggest that transient polymers are formed, the clinical significance of which is not known.

AB - We investigated the effect of streptokinase on determination of fibrinogen and related clottable proteins in an effort to assess the relative reliability of these determinations in monitoring patients being treated with streptokinase. Citrated plasma was incubated with and without streptokinase, then assayed for fibrinogen (I), fibrin(ogen) degradation products (II), total clottable protein (III), and plasminogen (IV). Values for I decreased rapidly. Values for III and IV generally paralleled those for I, values for III lacked adequate sensitivity. II, low initially, increased to 80 mg/L in 1-2 h. Soybean trypsin inhibitor effectively stabilized all these constituents (except IV) in streptokinase-treated plasma, and we recommend that this (or some other such) inhibitor of fibrinolysis be used routinely when specimens are collected for these determinations. Data are presented that suggest that transient polymers are formed, the clinical significance of which is not known.

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Effect of streptokinase on fibrinogen and related proteins in plasma

Abstract

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