Effect of various substances on colchicine uptake by cells sensitive and resistant to it

A. S. Serpinskaya, B. P. Kopnin, V. I. Gel'fand, V. A. Rozenblat, A. A. Stavrovskaya

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of various substances on the uptake of [3H]colchicine by L and L-53 cells, resistant to colcemid and colchicine was investigated. Vinblastin, to which L-53 cells possess cross resistance, increases accumulation of labeled colchicine in L cells threefold, and in L-53 cells fivefold. Substances lowering the ATP level in the cells (oligomycin, etc.) increase the uptake of colchicine by L and L-53 cells by 2-4 times. Colchicine uptake by resistant cells is increased in the presence of these substances more than in sensitive L cells. Lumicolchicine, a structural analog of colchicine which does not bind with tubulin, the protein of the microtubules, stimulates colchicine uptake approximately equally by L and L-53 cells.

Original languageEnglish (US)
Pages (from-to)1062-1065
Number of pages4
JournalBulletin of Experimental Biology and Medicine
Volume88
Issue number3
DOIs
StatePublished - Sep 1 1979

Keywords

  • Resistance
  • colchicine
  • permeability
  • uptake
  • vinblastin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Effect of various substances on colchicine uptake by cells sensitive and resistant to it'. Together they form a unique fingerprint.

Cite this