Abstract
In response to food deprivation, total myocardial lipoprotein lipase activity increased gradually over a period of 9 h. Although lipoprotein lipase exists in a functional and non-functional form in the myocardium, most of the increase in activity occurred in the functional (heparin-releasable) lipoprotein lipase fraction. The administration of colchicine, while having no effect on the increase seen in total lipoprotein lipase activity, did inhibit the increase in the functional fraction, while at the same time, caused a marked rise in the activity of the non-functional (non-releasable) fraction. In rats injected with colchicine after a 24-h fast, total lipoprotein lipase activity was not affected, but activity levels in the functional fraction declined while that in the non-functional fraction increased. These results suggest that the functional lipoprotein lipase is constantly being formed in sites not readily accessible to heparin (presumably the myocardial cells) and transported to its site of action, the surface of the endothelial cells of the capillaries. Cycloheximide administration to rats starved for 24 h caused a decline in activity in both the functional (half-life of about 2 h) and the non-functional (half-life of about 4 h) lipoprotein lipase fractions. These results suggest that the functional and non-functional lipoprotein lipase fractions may correspond to two distinct enzyme species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 394-400 |
| Number of pages | 7 |
| Journal | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
| Volume | 398 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 19 1975 |
Funding
This work was supported by Public I-Iealth Research Grants AM-16831 and A~-17046 and by the Chicago Heart Association. The authors are grateful to Drs G.S. Getz and LB Oscai for helpful discussion during the preparation of the manuscript.
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Endocrinology