Effects of fibrinogens on phase transitions in lipid model membrane systems

B. A. Cunningham; Ö Küçük; H. C. Kwaan; M. P. Westerman; D. Tracy; L. J. Lis

doi:10.1016/0009-3084(94)90011-6

Effects of fibrinogens on phase transitions in lipid model membrane systems

B. A. Cunningham, Ö Küçük^*, H. C. Kwaan, M. P. Westerman, D. Tracy, L. J. Lis

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

An abnormal fibrinogen that caused aggregation of red blood cells (RBC) in a patient with gangrene was examined by real-time X-ray diffraction to determine its effects on dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-,2-oleoylphosphatidylethanolamine (POPE) phase transitions. Similar studies were done with normal fibrinogen and results were compared. Both types of fibrinogen slightly increased the L_α → H_II phase transition temperature and the H_II phase parameters for POPE, while neither fibrinogen significantly affected the order-disordered acyl chain transitions in the lipid bilayer phase. However, fibrinogen differentially influenced the bilayer unit cell parameter of the gel and disordered bilayer and the gel state ripple phase. These results can be interpreted as indicating that fibrinogen has little effect on the balance of gel and disordered acyl chains in the lipid bilayer, but may influence membrane functions dependent on non-bilayer phases.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Chemistry and Physics of Lipids
Volume	72
Issue number	1
DOIs	https://doi.org/10.1016/0009-3084(94)90011-6
State	Published - Jun 24 1994

Keywords

Fibrinogen
Lipid model membrane
Phase transitions
X-ray diffraction

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Organic Chemistry
Cell Biology

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10.1016/0009-3084(94)90011-6

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title = "Effects of fibrinogens on phase transitions in lipid model membrane systems",

abstract = "An abnormal fibrinogen that caused aggregation of red blood cells (RBC) in a patient with gangrene was examined by real-time X-ray diffraction to determine its effects on dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-,2-oleoylphosphatidylethanolamine (POPE) phase transitions. Similar studies were done with normal fibrinogen and results were compared. Both types of fibrinogen slightly increased the Lα → HII phase transition temperature and the HII phase parameters for POPE, while neither fibrinogen significantly affected the order-disordered acyl chain transitions in the lipid bilayer phase. However, fibrinogen differentially influenced the bilayer unit cell parameter of the gel and disordered bilayer and the gel state ripple phase. These results can be interpreted as indicating that fibrinogen has little effect on the balance of gel and disordered acyl chains in the lipid bilayer, but may influence membrane functions dependent on non-bilayer phases.",

keywords = "Fibrinogen, Lipid model membrane, Phase transitions, X-ray diffraction",

author = "Cunningham, {B. A.} and {\"O} K{\"u}{\c c}{\"u}k and Kwaan, {H. C.} and Westerman, {M. P.} and D. Tracy and Lis, {L. J.}",

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T1 - Effects of fibrinogens on phase transitions in lipid model membrane systems

AU - Cunningham, B. A.

AU - Küçük, Ö

AU - Kwaan, H. C.

AU - Westerman, M. P.

AU - Tracy, D.

AU - Lis, L. J.

PY - 1994/6/24

Y1 - 1994/6/24

N2 - An abnormal fibrinogen that caused aggregation of red blood cells (RBC) in a patient with gangrene was examined by real-time X-ray diffraction to determine its effects on dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-,2-oleoylphosphatidylethanolamine (POPE) phase transitions. Similar studies were done with normal fibrinogen and results were compared. Both types of fibrinogen slightly increased the Lα → HII phase transition temperature and the HII phase parameters for POPE, while neither fibrinogen significantly affected the order-disordered acyl chain transitions in the lipid bilayer phase. However, fibrinogen differentially influenced the bilayer unit cell parameter of the gel and disordered bilayer and the gel state ripple phase. These results can be interpreted as indicating that fibrinogen has little effect on the balance of gel and disordered acyl chains in the lipid bilayer, but may influence membrane functions dependent on non-bilayer phases.

AB - An abnormal fibrinogen that caused aggregation of red blood cells (RBC) in a patient with gangrene was examined by real-time X-ray diffraction to determine its effects on dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-,2-oleoylphosphatidylethanolamine (POPE) phase transitions. Similar studies were done with normal fibrinogen and results were compared. Both types of fibrinogen slightly increased the Lα → HII phase transition temperature and the HII phase parameters for POPE, while neither fibrinogen significantly affected the order-disordered acyl chain transitions in the lipid bilayer phase. However, fibrinogen differentially influenced the bilayer unit cell parameter of the gel and disordered bilayer and the gel state ripple phase. These results can be interpreted as indicating that fibrinogen has little effect on the balance of gel and disordered acyl chains in the lipid bilayer, but may influence membrane functions dependent on non-bilayer phases.

KW - Fibrinogen

KW - Lipid model membrane

KW - Phase transitions

KW - X-ray diffraction

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Effects of fibrinogens on phase transitions in lipid model membrane systems

Abstract

Keywords

ASJC Scopus subject areas

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