Effects of inhibitors of nitric oxide synthase on isolated uteri of fasting rats

M. L. Campos, S. M. Casalino-Matsuda, J. A. Linares, A. Goldraij*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The effects of inhibitors of nitric oxide synthase on the glucose metabolism of uteri isolated from 4-day underfed rats were studied. In control rats receiving normal feeding, the addition of indomethacin (5 x 10-6 M); acetyl salicylic acid (10-4M); 400 μM of N(G)methyl-L-arginine, (L-NMMA) or 400 μM of sodium nitroprusside (SNP), does not modify the production of 14CO2 from U14C-glucose. On the contrary, in fasted rat uteri, indomethacin increases glucose oxidation significantly, while acetyl salicylic acid does not alter it. Also, the addition of L-NMMA has no effect. In another group of experiments, in the preparations containing indomethacin of uteri isolated from underfed rats, the addition of L-NMMA significantly changes the effect of indomethacin. Another inhibitor of nitric oxide synthase, Nωnitro-L-arginine-D-methyl ester (L-NAME), or hemoglobin (2 μg ml-1) a nitric oxide scavenger have the same effects while Nωnitro-L-arginine-D-methyl ester (D-NAME) does not. However (SNP), a nitric oxide donor, does not alter the production of 14CO2 in uteri isolated from fasted rats. These results show that in underfed rats, indomethacin increases glucose oxidation independently from its inhibiting effect on cyclooxygenase. Specific inhibitors of nitric oxide synthase can reverse this effect.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Issue number1
StatePublished - Jul 1998

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology


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