Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA

Chiang‐Tung ‐T Chang; Timothy C. Hain; James R. Hutton; James G. Wetmur

doi:10.1002/bip.1974.360130915

Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA

Chiang‐Tung ‐T Chang^*, Timothy C. Hain, James R. Hutton, James G. Wetmur

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The effect of solvent viscosity on DNA renaturation rates has been investigated as a function of temperature for a number of solvent systems. The results are all consistent with a microscopic viscosity limitation of the rate determining step. Rates of renaturation in perchlorate and quaternary ammonium salt solutions are also discussed. Increasing the macroscopic viscosity with dissolved neutral or anionic polymers increases, rather than decreases, renaturation rates due to the excluded volume of the dissolved polymers.

Original language	English (US)
Pages (from-to)	1847-1858
Number of pages	12
Journal	Biopolymers
Volume	13
Issue number	9
DOIs	https://doi.org/10.1002/bip.1974.360130915
State	Published - Jan 1 1974

ASJC Scopus subject areas

Biophysics
Biochemistry
Biomaterials
Organic Chemistry

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Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA

Abstract

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