Effects of oligohydramnios on lung growth and maturation in the fetal rat

Joseph A. Kitterman*, Cheryl J. Chapin, Jeff N. Vanderbilt, Nicolas F M Porta, Louis M. Scavo, Leland G. Dobbs, Robert Ertsey, Jon Goerke

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Oligohydramnios (OH) retards fetal lung growth by producing less lung distension than normal. To examine effects of decreased distension on fetal lung development, we produced OH in rats by puncture of uterus and fetal membranes at 16 days of gestation; fetuses were delivered at 21 or 22 days of gestation. Controls were position-matched littermates in the opposite uterine horn. OH lungs had lower weights and less DNA, protein, and water, but no differences in saturated phosphatidylcholine, surfactant proteins (SP)-A and -B, and mRNA for SP-A, -B, -C, and -D. To evaluate effects on epithelial differentiation, we used RTI40 and RTII70, proteins specific in lung to luminal surfaces of alveolar type I and II cells, respectively. At 22 days of gestation, OH lungs had less RTI40 mRNA (P < 0.05) and protein (P < 0.001), but RTII70 did not differ from controls. With OH, type I cells (in proportion to type II cells) covered less distal air space perimeter (P < 0.01), We conclude that OH, which retards lung growth, has little effect on surfactant and impedes formation of type I cells relative to type II cells.

Original languageEnglish (US)
Pages (from-to)L431-L439
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume282
Issue number3 26-3
DOIs
StatePublished - 2002

Keywords

  • Fetal lung development
  • Lung distension
  • Pulmonary epithelial differentiation
  • Pulmonary hypoplasia
  • Pulmonary surfactant

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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