Preincubation of BALB/c spleen cell cultures for 24 hr with phosphorylcholine (PC)-containing antigen together with antibody against the major idiotype (id) of anti-PC antibody renders them irreversibly unresponsive to subsequent stimulation with the antigen alone. In contrast, cultures preincubated for 24 hr with anti-id antibody, either alone or together with lipopolysaccharide (LPS), resulted in an anti-PC response comparable to that induced in control cultures incubated with mock anti-id antibody. After such a 24-hr preincubation with anti-id antibody and various PC-LPS conjugates possessing intact activity for polyclonal B-cell activation, the anti-PC response was inversely proportional to the epitope (PC) density on the LPS conjugates. In addition, similar preincubation of cultures with a non-mitogenic low dose of PC-LPS in the presence of anti-id antibody induced suppression of the anti-PC response as observed with a specific antigen. These results suggest that specific epitope delivers an additional tolerogenic signal during induction of B-cell suppression by anti-id antibody. This epitope effect cannot be replaced by, and is antagonistic to, the mitogenic signal of LPS in the course of B-cell inactivation.
- LPS, lipopolysaccharide from E. coli B55:05
- PBA, polyclonal B-cell activator
- PC, phos-phorylcholine
- PC-LPS, LPS conjugated with p-diazonium phenylphosphorylcholine
- PC-TG-LPS,LPS conjugated with p-diazonium phenylphosphoryl-choline-tyrosyl-glycine
- PnC, C-polysaccharide from R36a Streptococcus pneumoniae
- SRBC, sheep red blood cells
- T15id, idiotype of the TEPC-15 myeloma protein
- TNP, 2,4,6-trinitrophenyl.
- abrHEPES, N-2-hydroxy-piperazine-N-1-ethanesulfonate
ASJC Scopus subject areas
- Molecular Biology