Effects of phosphorylcholine-lipopolysaccharide conjugates on the induction of anti-idiotype-mediated B-cell tolerance

Patrick B. Massey; Byung S. Kim

doi:10.1016/0161-5890(85)90095-1

Effects of phosphorylcholine-lipopolysaccharide conjugates on the induction of anti-idiotype-mediated B-cell tolerance

Patrick B. Massey, Byung S. Kim^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

Preincubation of BALB/c spleen cell cultures for 24 hr with phosphorylcholine (PC)-containing antigen together with antibody against the major idiotype (id) of anti-PC antibody renders them irreversibly unresponsive to subsequent stimulation with the antigen alone. In contrast, cultures preincubated for 24 hr with anti-id antibody, either alone or together with lipopolysaccharide (LPS), resulted in an anti-PC response comparable to that induced in control cultures incubated with mock anti-id antibody. After such a 24-hr preincubation with anti-id antibody and various PC-LPS conjugates possessing intact activity for polyclonal B-cell activation, the anti-PC response was inversely proportional to the epitope (PC) density on the LPS conjugates. In addition, similar preincubation of cultures with a non-mitogenic low dose of PC-LPS in the presence of anti-id antibody induced suppression of the anti-PC response as observed with a specific antigen. These results suggest that specific epitope delivers an additional tolerogenic signal during induction of B-cell suppression by anti-id antibody. This epitope effect cannot be replaced by, and is antagonistic to, the mitogenic signal of LPS in the course of B-cell inactivation.

Original language	English (US)
Pages (from-to)	655-661
Number of pages	7
Journal	Molecular Immunology
Volume	22
Issue number	6
DOIs	https://doi.org/10.1016/0161-5890(85)90095-1
State	Published - Jun 1985

Funding

*Supported by grant AI 15446 from the U.S. Public Health Service. ?A11 correspondence should be addressed to: Dr Byung S. Kim, Department of Microbiology-immunology, Northwestern University Medical %hool, 303 -I?. Chicago Avenue, Chicago, IL 60611, U.S.A.

Keywords

LPS, lipopolysaccharide from E. coli B55:05
PBA, polyclonal B-cell activator
PC, phos-phorylcholine
PC-LPS, LPS conjugated with p-diazonium phenylphosphorylcholine
PC-TG-LPS,LPS conjugated with p-diazonium phenylphosphoryl-choline-tyrosyl-glycine
PnC, C-polysaccharide from R36a Streptococcus pneumoniae
SRBC, sheep red blood cells
T15id, idiotype of the TEPC-15 myeloma protein
TNP, 2,4,6-trinitrophenyl.
abrHEPES, N-2-hydroxy-piperazine-N-1-ethanesulfonate

ASJC Scopus subject areas

Molecular Biology
Immunology

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10.1016/0161-5890(85)90095-1

Cite this

@article{af1771b21b444056b160838d48ac6e80,

title = "Effects of phosphorylcholine-lipopolysaccharide conjugates on the induction of anti-idiotype-mediated B-cell tolerance",

abstract = "Preincubation of BALB/c spleen cell cultures for 24 hr with phosphorylcholine (PC)-containing antigen together with antibody against the major idiotype (id) of anti-PC antibody renders them irreversibly unresponsive to subsequent stimulation with the antigen alone. In contrast, cultures preincubated for 24 hr with anti-id antibody, either alone or together with lipopolysaccharide (LPS), resulted in an anti-PC response comparable to that induced in control cultures incubated with mock anti-id antibody. After such a 24-hr preincubation with anti-id antibody and various PC-LPS conjugates possessing intact activity for polyclonal B-cell activation, the anti-PC response was inversely proportional to the epitope (PC) density on the LPS conjugates. In addition, similar preincubation of cultures with a non-mitogenic low dose of PC-LPS in the presence of anti-id antibody induced suppression of the anti-PC response as observed with a specific antigen. These results suggest that specific epitope delivers an additional tolerogenic signal during induction of B-cell suppression by anti-id antibody. This epitope effect cannot be replaced by, and is antagonistic to, the mitogenic signal of LPS in the course of B-cell inactivation.",

keywords = "LPS, lipopolysaccharide from E. coli B55:05, PBA, polyclonal B-cell activator, PC, phos-phorylcholine, PC-LPS, LPS conjugated with p-diazonium phenylphosphorylcholine, PC-TG-LPS,LPS conjugated with p-diazonium phenylphosphoryl-choline-tyrosyl-glycine, PnC, C-polysaccharide from R36a Streptococcus pneumoniae, SRBC, sheep red blood cells, T15id, idiotype of the TEPC-15 myeloma protein, TNP, 2,4,6-trinitrophenyl., abrHEPES, N-2-hydroxy-piperazine-N-1-ethanesulfonate",

author = "Massey, {Patrick B.} and Kim, {Byung S.}",

note = "Funding Information: *Supported by grant AI 15446 from the U.S. Public Health Service. ?A11 correspondence should be addressed to: Dr Byung S. Kim, Department of Microbiology-immunology, Northwestern University Medical %hool, 303 -I?. Chicago Avenue, Chicago, IL 60611, U.S.A.",

year = "1985",

month = jun,

doi = "10.1016/0161-5890(85)90095-1",

language = "English (US)",

volume = "22",

pages = "655--661",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Ltd",

number = "6",

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T1 - Effects of phosphorylcholine-lipopolysaccharide conjugates on the induction of anti-idiotype-mediated B-cell tolerance

AU - Massey, Patrick B.

AU - Kim, Byung S.

N1 - Funding Information: *Supported by grant AI 15446 from the U.S. Public Health Service. ?A11 correspondence should be addressed to: Dr Byung S. Kim, Department of Microbiology-immunology, Northwestern University Medical %hool, 303 -I?. Chicago Avenue, Chicago, IL 60611, U.S.A.

PY - 1985/6

Y1 - 1985/6

N2 - Preincubation of BALB/c spleen cell cultures for 24 hr with phosphorylcholine (PC)-containing antigen together with antibody against the major idiotype (id) of anti-PC antibody renders them irreversibly unresponsive to subsequent stimulation with the antigen alone. In contrast, cultures preincubated for 24 hr with anti-id antibody, either alone or together with lipopolysaccharide (LPS), resulted in an anti-PC response comparable to that induced in control cultures incubated with mock anti-id antibody. After such a 24-hr preincubation with anti-id antibody and various PC-LPS conjugates possessing intact activity for polyclonal B-cell activation, the anti-PC response was inversely proportional to the epitope (PC) density on the LPS conjugates. In addition, similar preincubation of cultures with a non-mitogenic low dose of PC-LPS in the presence of anti-id antibody induced suppression of the anti-PC response as observed with a specific antigen. These results suggest that specific epitope delivers an additional tolerogenic signal during induction of B-cell suppression by anti-id antibody. This epitope effect cannot be replaced by, and is antagonistic to, the mitogenic signal of LPS in the course of B-cell inactivation.

AB - Preincubation of BALB/c spleen cell cultures for 24 hr with phosphorylcholine (PC)-containing antigen together with antibody against the major idiotype (id) of anti-PC antibody renders them irreversibly unresponsive to subsequent stimulation with the antigen alone. In contrast, cultures preincubated for 24 hr with anti-id antibody, either alone or together with lipopolysaccharide (LPS), resulted in an anti-PC response comparable to that induced in control cultures incubated with mock anti-id antibody. After such a 24-hr preincubation with anti-id antibody and various PC-LPS conjugates possessing intact activity for polyclonal B-cell activation, the anti-PC response was inversely proportional to the epitope (PC) density on the LPS conjugates. In addition, similar preincubation of cultures with a non-mitogenic low dose of PC-LPS in the presence of anti-id antibody induced suppression of the anti-PC response as observed with a specific antigen. These results suggest that specific epitope delivers an additional tolerogenic signal during induction of B-cell suppression by anti-id antibody. This epitope effect cannot be replaced by, and is antagonistic to, the mitogenic signal of LPS in the course of B-cell inactivation.

KW - LPS, lipopolysaccharide from E. coli B55:05

KW - PBA, polyclonal B-cell activator

KW - PC, phos-phorylcholine

KW - PC-LPS, LPS conjugated with p-diazonium phenylphosphorylcholine

KW - PC-TG-LPS,LPS conjugated with p-diazonium phenylphosphoryl-choline-tyrosyl-glycine

KW - PnC, C-polysaccharide from R36a Streptococcus pneumoniae

KW - SRBC, sheep red blood cells

KW - T15id, idiotype of the TEPC-15 myeloma protein

KW - TNP, 2,4,6-trinitrophenyl.

KW - abrHEPES, N-2-hydroxy-piperazine-N-1-ethanesulfonate

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DO - 10.1016/0161-5890(85)90095-1

M3 - Article

C2 - 2410779

AN - SCOPUS:0021809327

SN - 0161-5890

VL - 22

SP - 655

EP - 661

JO - Molecular Immunology

JF - Molecular Immunology

IS - 6

ER -

Effects of phosphorylcholine-lipopolysaccharide conjugates on the induction of anti-idiotype-mediated B-cell tolerance

Abstract

Funding

Keywords

ASJC Scopus subject areas

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