Effects of Prolonged Depolarization on the Nicotinic Acetylcholine Receptors of PC12 Cells

Eleanor M. DeLorme, Richard McGee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Abstract: To determine whether prolonged depolarization and/or changes in intracellular Ca2+ concentrations stimulate adaptive responses of neuronal nicotinic acetylcholine receptors, PC12 pheochromocytoma cells were grown in medium containing various concentrations of K+. Nicotinic receptor function was determined as carbachol‐stimulated uptake of 86Rb+. Cells were exposed to 50 mM K+ for up to 4 days and then allowed to repolarize for 60 min. Under these conditions, no changes in basal or carbachol‐stimulated uptake of 86Rb+ were observed. Furthermore, neither the time course of carbachol‐stimulated uptake or the carbachol concentration dependence of 86Rb+ uptake was altered. Finally, concurrent depolarization did not affect the functional down‐regulation produced by chronic exposure of the cells to carbachol. Thus, neuronal nicotinic acetylcholine receptors on PC12 cells do not appear to be regulated by depolarization or prolonged elevation of the intracellular Ca2+ level.

Original languageEnglish (US)
Pages (from-to)1248-1252
Number of pages5
JournalJournal of Neurochemistry
Volume50
Issue number4
DOIs
StatePublished - Jan 1 1988

Keywords

  • Acetylcholine receptors
  • Depolarization
  • Membrane potential
  • Nicotinic receptors
  • PC 12 cells
  • Pheochromocytoma cells
  • Regulation

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Cellular and Molecular Neuroscience

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