Effects of the antiestrogens tamoxifen and clomiphene on bone resorption in vitro

Pamela J. Stewart*, Paula H. Stern

*Corresponding author for this work

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

The in vitro effect of the nonsteroidal antiestrogens tamoxifen (TAM) and clomiphene (CLO) on bone resportion was investigated. TAM (100 μM) and CLO (100 μM) completely blocked PTH (2 nM)-induced resorption; 10 μM TAM was ineffective in blocking resorption, while 40–50 μM partially inhibited the response. TAM (100 μM) also completely blocked prostaglandin E2 (30 nM)- and 1,25-dihydroxy vitamin D3 (0.5 nM)-induced bone resorption. A 16-h pretreatment with TAM blocked subsequent stimulation of resorption by PTH, whereas 3.5- or 7-h pretreatment with antiestrogen was ineffective in blocking the response. Both protein and DNA syntheses were inhibited by continuous treatment (48 h) with the antiestrogens. When antiestrogen-pretreated (16 h) bones were transferred to fresh medium not containing antiestrogen, protein and DNA syntheses recovered to approximately half the control (nonantiestrogen- treated) values within 48 h. Bone resorption, however, was still completely inhibited even though macromolecular synthesis had substantially recovered. Thus, mechanisms other than macromolecular synthesis inhibition could be involved in the inhibition of bone resorption by the nonsteroidal antiestrogens TAM and CLO.

Original languageEnglish (US)
Pages (from-to)125-131
Number of pages7
JournalEndocrinology
Volume118
Issue number1
DOIs
StatePublished - Jan 1986

ASJC Scopus subject areas

  • Endocrinology

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