Effects of urine on the functional quality of human spermatozoa

D. Chen, M. J. Scobey, R. S. Jeyendran*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Objective: To determine the effect of urine on sperm function and to elucidate the reason by which it may be deleterious. Design: Prospective controlled in vitro study. Interactions: Sperm were exposed to various concentrations of pooled urine in Ham's F-10 Medium supplemented with 3.5 g% bovine serum albumin (GIBCO, Grand Island, NY) and sperm exposed to Ham's F- 10 Medium with pH and osmolality adjusted to mimic that of the urine (pH 5.4; 520 mOsm). Main Outcome Measure: Sperm motility and the ability of sperm to penetrate and migrate into mucus and to penetrate hamster oocytes were determined. Results: All sperm variables studied were significantly decreased when the sperm were exposed to ≥40% urine. Potential of hydrogen- and osmolality-adjusted Ham's F-10 did not significantly affect the sperm function. Conclusion: Urine is deleterious to sperm function and may involve other biochemical factors that are unrelated to pH and osmolality of urine.

Original languageEnglish (US)
Pages (from-to)1216-1217
Number of pages2
JournalFertility and Sterility
Issue number6
StatePublished - 1995


  • Urine
  • function
  • migration
  • motility
  • mucus
  • oocytes
  • penetration
  • sperm

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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