Effects of urine on the functional quality of human spermatozoa

D. Chen; M. J. Scobey; R. S. Jeyendran

doi:10.1016/s0015-0282(16)57990-4

Effects of urine on the functional quality of human spermatozoa

D. Chen, M. J. Scobey, R. S. Jeyendran^*

^*Corresponding author for this work

Physical Medicine and Rehabilitation

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Objective: To determine the effect of urine on sperm function and to elucidate the reason by which it may be deleterious. Design: Prospective controlled in vitro study. Interactions: Sperm were exposed to various concentrations of pooled urine in Ham's F-10 Medium supplemented with 3.5 g% bovine serum albumin (GIBCO, Grand Island, NY) and sperm exposed to Ham's F- 10 Medium with pH and osmolality adjusted to mimic that of the urine (pH 5.4; 520 mOsm). Main Outcome Measure: Sperm motility and the ability of sperm to penetrate and migrate into mucus and to penetrate hamster oocytes were determined. Results: All sperm variables studied were significantly decreased when the sperm were exposed to ≥40% urine. Potential of hydrogen- and osmolality-adjusted Ham's F-10 did not significantly affect the sperm function. Conclusion: Urine is deleterious to sperm function and may involve other biochemical factors that are unrelated to pH and osmolality of urine.

Original language	English (US)
Pages (from-to)	1216-1217
Number of pages	2
Journal	Fertility and Sterility
Volume	64
Issue number	6
DOIs	https://doi.org/10.1016/s0015-0282(16)57990-4
State	Published - 1995

Keywords

Urine
function
migration
motility
mucus
oocytes
penetration
sperm

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1016/s0015-0282(16)57990-4

Cite this

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title = "Effects of urine on the functional quality of human spermatozoa",

abstract = "Objective: To determine the effect of urine on sperm function and to elucidate the reason by which it may be deleterious. Design: Prospective controlled in vitro study. Interactions: Sperm were exposed to various concentrations of pooled urine in Ham's F-10 Medium supplemented with 3.5 g% bovine serum albumin (GIBCO, Grand Island, NY) and sperm exposed to Ham's F- 10 Medium with pH and osmolality adjusted to mimic that of the urine (pH 5.4; 520 mOsm). Main Outcome Measure: Sperm motility and the ability of sperm to penetrate and migrate into mucus and to penetrate hamster oocytes were determined. Results: All sperm variables studied were significantly decreased when the sperm were exposed to ≥40% urine. Potential of hydrogen- and osmolality-adjusted Ham's F-10 did not significantly affect the sperm function. Conclusion: Urine is deleterious to sperm function and may involve other biochemical factors that are unrelated to pH and osmolality of urine.",

keywords = "Urine, function, migration, motility, mucus, oocytes, penetration, sperm",

author = "D. Chen and Scobey, {M. J.} and Jeyendran, {R. S.}",

note = "Funding Information: Received December 27, 1994; revised and accepted May 17, 1995. * Supported in part by the Dr. Ralph and Marion C. Falk Medical Trust, Chicago, Illinois. t Department of Physical Medicine and Rehabilitation Northwestern University Medical School. :j: Andrology Laboratory Services, Inc. § Department of Obstetrics and Gynecology, Northwestern University Medical School. II Reprint requests: Rajasingam Jeyendran, D.V.M., Ph.D., 680 North Lake Shore Drive, Suite 1025, Chicago, Illinois 60611 (FAX: 312-335-0076).",

year = "1995",

doi = "10.1016/s0015-0282(16)57990-4",

language = "English (US)",

volume = "64",

pages = "1216--1217",

journal = "Fertility and Sterility",

issn = "0015-0282",

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T1 - Effects of urine on the functional quality of human spermatozoa

AU - Chen, D.

AU - Scobey, M. J.

AU - Jeyendran, R. S.

N1 - Funding Information: Received December 27, 1994; revised and accepted May 17, 1995. * Supported in part by the Dr. Ralph and Marion C. Falk Medical Trust, Chicago, Illinois. t Department of Physical Medicine and Rehabilitation Northwestern University Medical School. :j: Andrology Laboratory Services, Inc. § Department of Obstetrics and Gynecology, Northwestern University Medical School. II Reprint requests: Rajasingam Jeyendran, D.V.M., Ph.D., 680 North Lake Shore Drive, Suite 1025, Chicago, Illinois 60611 (FAX: 312-335-0076).

PY - 1995

Y1 - 1995

N2 - Objective: To determine the effect of urine on sperm function and to elucidate the reason by which it may be deleterious. Design: Prospective controlled in vitro study. Interactions: Sperm were exposed to various concentrations of pooled urine in Ham's F-10 Medium supplemented with 3.5 g% bovine serum albumin (GIBCO, Grand Island, NY) and sperm exposed to Ham's F- 10 Medium with pH and osmolality adjusted to mimic that of the urine (pH 5.4; 520 mOsm). Main Outcome Measure: Sperm motility and the ability of sperm to penetrate and migrate into mucus and to penetrate hamster oocytes were determined. Results: All sperm variables studied were significantly decreased when the sperm were exposed to ≥40% urine. Potential of hydrogen- and osmolality-adjusted Ham's F-10 did not significantly affect the sperm function. Conclusion: Urine is deleterious to sperm function and may involve other biochemical factors that are unrelated to pH and osmolality of urine.

AB - Objective: To determine the effect of urine on sperm function and to elucidate the reason by which it may be deleterious. Design: Prospective controlled in vitro study. Interactions: Sperm were exposed to various concentrations of pooled urine in Ham's F-10 Medium supplemented with 3.5 g% bovine serum albumin (GIBCO, Grand Island, NY) and sperm exposed to Ham's F- 10 Medium with pH and osmolality adjusted to mimic that of the urine (pH 5.4; 520 mOsm). Main Outcome Measure: Sperm motility and the ability of sperm to penetrate and migrate into mucus and to penetrate hamster oocytes were determined. Results: All sperm variables studied were significantly decreased when the sperm were exposed to ≥40% urine. Potential of hydrogen- and osmolality-adjusted Ham's F-10 did not significantly affect the sperm function. Conclusion: Urine is deleterious to sperm function and may involve other biochemical factors that are unrelated to pH and osmolality of urine.

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KW - oocytes

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Effects of urine on the functional quality of human spermatozoa

Abstract

Keywords

ASJC Scopus subject areas

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