Effects of vasoactive intestinal contractor (VIC) and endothelin on intracellular calcium level in neuroblastoma NG108-15 cells

Tao Fu, Wei Chang, Norio Ishida, Kaname Saida, Youji Mitsui, Yukio Okano, Yoshinori Nozawa

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Effects on [Ca2+]i levels of endothelin-1 (ET) and vasoactive intestinal contractor peptide (VIC), which is a novel member of the endothelin family, were examined in fura 2-loaded neuroblastoma NG108-15 cells. VIC was found to be a very effective stimulus for intracellular Ca2+ mobilization and to be more potent than ET. Intracellular calcium response to sequential addition of two stimulants exhibited the homologous desensitization of either ET or VIC, but no heterologous desensitization between ET and VIC. This indicates evidence suggesting that these two peptides act through distinct receptors.

Original languageEnglish (US)
Pages (from-to)351-353
Number of pages3
JournalFEBS Letters
Volume257
Issue number2
DOIs
StatePublished - Nov 6 1989

Keywords

  • (Neuroblastoma NG108-15)
  • Ca level, intracellular
  • Endothelin-1
  • Fura 2
  • Inositol 1,4,5-triphosphate
  • Vasoactive intestinal contractor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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