Efficient in vitro expansion of JC virus-specific CD8+ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy

Angela Marzocchetti, Marco Lima, Troy Tompkins, Daniel G. Kavanagh, Rajesh T. Gandhi, David W. O'Neill, Nina Bhardwaj, Igor J. Koralnik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the brain caused by JC virus (JCV) for which there is no cure. PML patients who have JCV-specific CD8+ cytotoxic T lymphocytes (CTL) in their blood have a better clinical outcome. We compared JCV-specific CTL responses in vitro elicited either by JCV peptide-loaded dendritic cells (DC) or by direct peptide stimulation of lymphocytes from 20 HLA-A*0201+ healthy controls, HIV+ and PML patients. JCV peptide-loaded DC elicited a stronger CTL expansion in 13/15 responders. DC can induce a potent JCV-specific CTL response in vitro, and may constitute a promising approach for PML immunotherapy.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalVirology
Volume383
Issue number2
DOIs
StatePublished - Jan 20 2009

Keywords

  • Dendritic cells
  • JC virus
  • Progressive multifocal leukoencephalopathy

ASJC Scopus subject areas

  • Virology

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