Efficient in vitro expansion of JC virus-specific CD8+ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy

Angela Marzocchetti; Marco Lima; Troy Tompkins; Daniel G. Kavanagh; Rajesh T. Gandhi; David W. O'Neill; Nina Bhardwaj; Igor J. Koralnik

doi:10.1016/j.virol.2008.10.046

Efficient in vitro expansion of JC virus-specific CD8⁺ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy

Angela Marzocchetti, Marco Lima, Troy Tompkins, Daniel G. Kavanagh, Rajesh T. Gandhi, David W. O'Neill, Nina Bhardwaj, Igor J. Koralnik^*

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the brain caused by JC virus (JCV) for which there is no cure. PML patients who have JCV-specific CD8⁺ cytotoxic T lymphocytes (CTL) in their blood have a better clinical outcome. We compared JCV-specific CTL responses in vitro elicited either by JCV peptide-loaded dendritic cells (DC) or by direct peptide stimulation of lymphocytes from 20 HLA-A*0201⁺ healthy controls, HIV⁺ and PML patients. JCV peptide-loaded DC elicited a stronger CTL expansion in 13/15 responders. DC can induce a potent JCV-specific CTL response in vitro, and may constitute a promising approach for PML immunotherapy.

Original language	English (US)
Pages (from-to)	173-177
Number of pages	5
Journal	Virology
Volume	383
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.10.046
State	Published - Jan 20 2009

Funding

This work was supported in part by Public Health Service Grants R01 NS/AI 041198 NS 047029, and K24 NS 060950 to IJK.

Keywords

Dendritic cells
JC virus
Progressive multifocal leukoencephalopathy

ASJC Scopus subject areas

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.virol.2008.10.046

Cite this

Marzocchetti, A., Lima, M., Tompkins, T., Kavanagh, D. G., Gandhi, R. T., O'Neill, D. W., Bhardwaj, N., & Koralnik, I. J. (2009). Efficient in vitro expansion of JC virus-specific CD8⁺ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy. Virology, 383(2), 173-177. https://doi.org/10.1016/j.virol.2008.10.046

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abstract = "Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the brain caused by JC virus (JCV) for which there is no cure. PML patients who have JCV-specific CD8+ cytotoxic T lymphocytes (CTL) in their blood have a better clinical outcome. We compared JCV-specific CTL responses in vitro elicited either by JCV peptide-loaded dendritic cells (DC) or by direct peptide stimulation of lymphocytes from 20 HLA-A*0201+ healthy controls, HIV+ and PML patients. JCV peptide-loaded DC elicited a stronger CTL expansion in 13/15 responders. DC can induce a potent JCV-specific CTL response in vitro, and may constitute a promising approach for PML immunotherapy.",

keywords = "Dendritic cells, JC virus, Progressive multifocal leukoencephalopathy",

author = "Angela Marzocchetti and Marco Lima and Troy Tompkins and Kavanagh, {Daniel G.} and Gandhi, {Rajesh T.} and O'Neill, {David W.} and Nina Bhardwaj and Koralnik, {Igor J.}",

note = "Funding Information: This work was supported in part by Public Health Service Grants R01 NS/AI 041198 NS 047029, and K24 NS 060950 to IJK.",

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Marzocchetti, A, Lima, M, Tompkins, T, Kavanagh, DG, Gandhi, RT, O'Neill, DW, Bhardwaj, N & Koralnik, IJ 2009, 'Efficient in vitro expansion of JC virus-specific CD8⁺ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy', Virology, vol. 383, no. 2, pp. 173-177. https://doi.org/10.1016/j.virol.2008.10.046

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AU - Lima, Marco

AU - Tompkins, Troy

AU - Kavanagh, Daniel G.

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AU - Bhardwaj, Nina

AU - Koralnik, Igor J.

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