EGF receptor binding studies in endometrial cell culture

Joseph M. Sorrentino; Mary J.C. Hendrix

doi:10.1016/0024-3205(84)90577-0

EGF receptor binding studies in endometrial cell culture

Joseph M. Sorrentino^*, Mary J.C. Hendrix

^*Corresponding author for this work

Robert H. Lurie Comprehensive Cancer Center

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Guinea pig endometrial cells were isolated and maintained in cell culture. Our investigation of these cultures for the presence of epidermal growth factor (EGF) binding activity demonstrated that these cells possess EGF receptors. Upon the addition of EGF to growing cell cultures, an increase in the rate of cell growth resulted along with a higher saturation density. The binding of EGF to these cells is saturable at 40-50 ng/ml., and the receptors demonstrate "down-regulation" in response to hormonal challenge at 37°C.

Original language	English (US)
Pages (from-to)	1769-1774
Number of pages	6
Journal	Life Sciences
Volume	34
Issue number	18
DOIs	https://doi.org/10.1016/0024-3205(84)90577-0
State	Published - Apr 30 1984

Funding

The EGF used in these studies was a gift from Dr. Harvey Herschman, UCLA. The authors wish to thank Miss Beth Elrod for typing the manuscript. This work was supported by ACS Institutional Research Grant # IN-1105, Arizona Foundation, and NIH Grant R01 HLDH 28850-02.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(84)90577-0

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abstract = "Guinea pig endometrial cells were isolated and maintained in cell culture. Our investigation of these cultures for the presence of epidermal growth factor (EGF) binding activity demonstrated that these cells possess EGF receptors. Upon the addition of EGF to growing cell cultures, an increase in the rate of cell growth resulted along with a higher saturation density. The binding of EGF to these cells is saturable at 40-50 ng/ml., and the receptors demonstrate {"}down-regulation{"} in response to hormonal challenge at 37°C.",

author = "Sorrentino, {Joseph M.} and Hendrix, {Mary J.C.}",

note = "Funding Information: The EGF used in these studies was a gift from Dr. Harvey Herschman, UCLA. The authors wish to thank Miss Beth Elrod for typing the manuscript. This work was supported by ACS Institutional Research Grant # IN-1105, Arizona Foundation, and NIH Grant R01 HLDH 28850-02.",

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AU - Hendrix, Mary J.C.

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PY - 1984/4/30

Y1 - 1984/4/30

N2 - Guinea pig endometrial cells were isolated and maintained in cell culture. Our investigation of these cultures for the presence of epidermal growth factor (EGF) binding activity demonstrated that these cells possess EGF receptors. Upon the addition of EGF to growing cell cultures, an increase in the rate of cell growth resulted along with a higher saturation density. The binding of EGF to these cells is saturable at 40-50 ng/ml., and the receptors demonstrate "down-regulation" in response to hormonal challenge at 37°C.

AB - Guinea pig endometrial cells were isolated and maintained in cell culture. Our investigation of these cultures for the presence of epidermal growth factor (EGF) binding activity demonstrated that these cells possess EGF receptors. Upon the addition of EGF to growing cell cultures, an increase in the rate of cell growth resulted along with a higher saturation density. The binding of EGF to these cells is saturable at 40-50 ng/ml., and the receptors demonstrate "down-regulation" in response to hormonal challenge at 37°C.

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EGF receptor binding studies in endometrial cell culture

Abstract

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ASJC Scopus subject areas

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