Abstract
Guinea pig endometrial cells were isolated and maintained in cell culture. Our investigation of these cultures for the presence of epidermal growth factor (EGF) binding activity demonstrated that these cells possess EGF receptors. Upon the addition of EGF to growing cell cultures, an increase in the rate of cell growth resulted along with a higher saturation density. The binding of EGF to these cells is saturable at 40-50 ng/ml., and the receptors demonstrate "down-regulation" in response to hormonal challenge at 37°C.
Original language | English (US) |
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Pages (from-to) | 1769-1774 |
Number of pages | 6 |
Journal | Life Sciences |
Volume | 34 |
Issue number | 18 |
DOIs | |
State | Published - Apr 30 1984 |
Funding
The EGF used in these studies was a gift from Dr. Harvey Herschman, UCLA. The authors wish to thank Miss Beth Elrod for typing the manuscript. This work was supported by ACS Institutional Research Grant # IN-1105, Arizona Foundation, and NIH Grant R01 HLDH 28850-02.
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- Pharmacology, Toxicology and Pharmaceutics(all)