Electro-acupuncture improves survival and migration of transplanted neural stem cells in injured spinal cord in rats

Ya Yun Chen; Wei Zhang; Yu Lin Chen; Shui Jun Chen; Hongxin Dong; Yuan Shan Zeng

doi:10.3727/036012908803861212

Electro-acupuncture improves survival and migration of transplanted neural stem cells in injured spinal cord in rats

Ya Yun Chen, Wei Zhang, Yu Lin Chen, Shui Jun Chen, Hongxin Dong, Yuan Shan Zeng^*

^*Corresponding author for this work

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

This study investigated whether electro-acupuncture (EA) would improve the survival and migration of neural stem cells (NSCs) transplanted in injured spinal cord as well as the potential mechanisms. T10 spinal cord segments of 50 adult Sprague-Dawley (SD) rats were completely transected, and then NSCs were immediately transplanted into the transected site of the experimental animals, while control animals were sham operated without transplantation. Five days post-operation, electro-acupuncture treatment on GV9 (Zhiyang), GV6 (Jizhong), GV2 (Yaoshu) and GV1 (Changqiang) acupoints was applied for 14 days (EA+NSCs 14d) and 30 days (EA+NSCs 30d). ELISA and immunohistochemical staining were used to assess the content of neurotrophine-3 (NT-3) and the characteristics of transplanted NSCs. We found that the number of transplanted NSCs the survived in EA+NSCs 14d group was significantly increased as compared to that of the NSCs30d group (5825.20 ± 819.01 vs 4781.40 ± 500.49, P<0.05). Immunostaining indicated that some transplanted NSCs developed into microtubule association protein 2 (MAP2) positive cells and many of them developed into glial fibrillary acidic protein (GFAP) positive cells in the NSCs30d group. Further, the migration length of transplanted NSCs toward caudal tissue in the injured site was longer in the EA+NSCs30d group than that in NSCs30d group (5.98±0.79 mm vs 3.96±1.72 mm; P<0.05). Also NT-3 in injured spinal cord tissue was 23% increased in the EA+NSCs 14d group. These results suggest that the combination of EA and NSCs improves the survival and migration of NSCs in injured spinal cord in rats.

Original language	English (US)
Pages (from-to)	19-31
Number of pages	13
Journal	Acupuncture and Electro-Therapeutics Research
Volume	33
Issue number	1-2
DOIs	https://doi.org/10.3727/036012908803861212
State	Published - 2008

Keywords

Cell migratory orientation
Electro-acupuncture
Nerotrophine-3
Neural stem cells
Spinal cord injury
Stem cell transplant

ASJC Scopus subject areas

Anesthesiology and Pain Medicine
Complementary and alternative medicine
General Neuroscience

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10.3727/036012908803861212

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title = "Electro-acupuncture improves survival and migration of transplanted neural stem cells in injured spinal cord in rats",

abstract = "This study investigated whether electro-acupuncture (EA) would improve the survival and migration of neural stem cells (NSCs) transplanted in injured spinal cord as well as the potential mechanisms. T10 spinal cord segments of 50 adult Sprague-Dawley (SD) rats were completely transected, and then NSCs were immediately transplanted into the transected site of the experimental animals, while control animals were sham operated without transplantation. Five days post-operation, electro-acupuncture treatment on GV9 (Zhiyang), GV6 (Jizhong), GV2 (Yaoshu) and GV1 (Changqiang) acupoints was applied for 14 days (EA+NSCs 14d) and 30 days (EA+NSCs 30d). ELISA and immunohistochemical staining were used to assess the content of neurotrophine-3 (NT-3) and the characteristics of transplanted NSCs. We found that the number of transplanted NSCs the survived in EA+NSCs 14d group was significantly increased as compared to that of the NSCs30d group (5825.20 ± 819.01 vs 4781.40 ± 500.49, P<0.05). Immunostaining indicated that some transplanted NSCs developed into microtubule association protein 2 (MAP2) positive cells and many of them developed into glial fibrillary acidic protein (GFAP) positive cells in the NSCs30d group. Further, the migration length of transplanted NSCs toward caudal tissue in the injured site was longer in the EA+NSCs30d group than that in NSCs30d group (5.98±0.79 mm vs 3.96±1.72 mm; P<0.05). Also NT-3 in injured spinal cord tissue was 23% increased in the EA+NSCs 14d group. These results suggest that the combination of EA and NSCs improves the survival and migration of NSCs in injured spinal cord in rats.",

keywords = "Cell migratory orientation, Electro-acupuncture, Nerotrophine-3, Neural stem cells, Spinal cord injury, Stem cell transplant",

author = "Chen, {Ya Yun} and Wei Zhang and Chen, {Yu Lin} and Chen, {Shui Jun} and Hongxin Dong and Zeng, {Yuan Shan}",

year = "2008",

doi = "10.3727/036012908803861212",

language = "English (US)",

volume = "33",

pages = "19--31",

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TY - JOUR

T1 - Electro-acupuncture improves survival and migration of transplanted neural stem cells in injured spinal cord in rats

AU - Chen, Ya Yun

AU - Zhang, Wei

AU - Chen, Yu Lin

AU - Chen, Shui Jun

AU - Dong, Hongxin

AU - Zeng, Yuan Shan

PY - 2008

Y1 - 2008

N2 - This study investigated whether electro-acupuncture (EA) would improve the survival and migration of neural stem cells (NSCs) transplanted in injured spinal cord as well as the potential mechanisms. T10 spinal cord segments of 50 adult Sprague-Dawley (SD) rats were completely transected, and then NSCs were immediately transplanted into the transected site of the experimental animals, while control animals were sham operated without transplantation. Five days post-operation, electro-acupuncture treatment on GV9 (Zhiyang), GV6 (Jizhong), GV2 (Yaoshu) and GV1 (Changqiang) acupoints was applied for 14 days (EA+NSCs 14d) and 30 days (EA+NSCs 30d). ELISA and immunohistochemical staining were used to assess the content of neurotrophine-3 (NT-3) and the characteristics of transplanted NSCs. We found that the number of transplanted NSCs the survived in EA+NSCs 14d group was significantly increased as compared to that of the NSCs30d group (5825.20 ± 819.01 vs 4781.40 ± 500.49, P<0.05). Immunostaining indicated that some transplanted NSCs developed into microtubule association protein 2 (MAP2) positive cells and many of them developed into glial fibrillary acidic protein (GFAP) positive cells in the NSCs30d group. Further, the migration length of transplanted NSCs toward caudal tissue in the injured site was longer in the EA+NSCs30d group than that in NSCs30d group (5.98±0.79 mm vs 3.96±1.72 mm; P<0.05). Also NT-3 in injured spinal cord tissue was 23% increased in the EA+NSCs 14d group. These results suggest that the combination of EA and NSCs improves the survival and migration of NSCs in injured spinal cord in rats.

AB - This study investigated whether electro-acupuncture (EA) would improve the survival and migration of neural stem cells (NSCs) transplanted in injured spinal cord as well as the potential mechanisms. T10 spinal cord segments of 50 adult Sprague-Dawley (SD) rats were completely transected, and then NSCs were immediately transplanted into the transected site of the experimental animals, while control animals were sham operated without transplantation. Five days post-operation, electro-acupuncture treatment on GV9 (Zhiyang), GV6 (Jizhong), GV2 (Yaoshu) and GV1 (Changqiang) acupoints was applied for 14 days (EA+NSCs 14d) and 30 days (EA+NSCs 30d). ELISA and immunohistochemical staining were used to assess the content of neurotrophine-3 (NT-3) and the characteristics of transplanted NSCs. We found that the number of transplanted NSCs the survived in EA+NSCs 14d group was significantly increased as compared to that of the NSCs30d group (5825.20 ± 819.01 vs 4781.40 ± 500.49, P<0.05). Immunostaining indicated that some transplanted NSCs developed into microtubule association protein 2 (MAP2) positive cells and many of them developed into glial fibrillary acidic protein (GFAP) positive cells in the NSCs30d group. Further, the migration length of transplanted NSCs toward caudal tissue in the injured site was longer in the EA+NSCs30d group than that in NSCs30d group (5.98±0.79 mm vs 3.96±1.72 mm; P<0.05). Also NT-3 in injured spinal cord tissue was 23% increased in the EA+NSCs 14d group. These results suggest that the combination of EA and NSCs improves the survival and migration of NSCs in injured spinal cord in rats.

KW - Cell migratory orientation

KW - Electro-acupuncture

KW - Nerotrophine-3

KW - Neural stem cells

KW - Spinal cord injury

KW - Stem cell transplant

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Electro-acupuncture improves survival and migration of transplanted neural stem cells in injured spinal cord in rats

Abstract

Keywords

ASJC Scopus subject areas

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