Abstract
Implantable cell therapies and tissue transplants require sufficient oxygen supply to function and are limited by a delay or lack of vascularization from the transplant host. Previous exogenous oxygenation strategies have been bulky and had limited oxygen production or regulation. Here, we show an electrocatalytic approach that enables bioelectronic control of oxygen generation in complex cellular environments to sustain engineered cell viability and therapy under hypoxic stress and at high cell densities. We find that nanostructured sputtered iridium oxide serves as an ideal catalyst for oxygen evolution reaction at neutral pH. We demonstrate that this approach exhibits a lower oxygenation onset and selective oxygen production without evolution of toxic byproducts. We show that this electrocatalytic on site oxygenator can sustain high cell loadings (>60k cells/mm3) in hypoxic conditions in vitro and in vivo. Our results showcase that exogenous oxygen production devices can be readily integrated into bioelectronic platforms, enabling high cell loadings in smaller devices with broad applicability.
| Original language | English (US) |
|---|---|
| Article number | 7019 |
| Journal | Nature communications |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 2023 |
Funding
This material is based on research sponsored by 711 Human Performance Wing (HPW) and Defense Advanced Research Projects Agency (DARPA) under agreement number FA8650-21-1-7119. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright notation thereon. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of 711 Human Performance Wing (HPW) and Defense Advanced Research Projects Agency (DARPA) or the U.S. Government. T.C.-K., S. Jo, and I.L. acknowledge support from Carnegie Mellon University Department of Materials Science and Engineering Materials Characterization Facility supported by Grant MCF-677785. T.C.-K., S. Jo, and I.L. would like to thank Dr. Loren Rieth for helpful discussion about SIROF and Mark Weiler and Dr. Matthew Moneck from the Claire and John Bertucci Nanotechnology Laboratory for assistance with SIROF deposition. J.R., A. Surendran, and X.J. acknowledge Northwestern University’s NUANCE Center, which has received support from the SHyNE Resource (NSF ECCS-2025633), the IIN, and Northwestern’s MRSEC program (NSF DMR-1720139). J.R. and A. Surendran acknowledge support from Analytical BioNanoTechnology Equipment Core (ANTEC) supported by the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-2025633) for material characterization and Center for Advanced Microscopy/Nikon Imaging Center (CAM) supported by CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center for confocal imaging. J.R., T.C.-K., A. Surendran, and I.L. acknowledge support from Blackrock Neurotech for SIROF, and wire-bundle bonding for the in vivo experiments. D.J.S. was supported by the National Heart, Lung, and Blood Institute of the National Institutes of Health under Award Number K99HL155777. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. This material is based on research sponsored by 711 Human Performance Wing (HPW) and Defense Advanced Research Projects Agency (DARPA) under agreement number FA8650-21-1-7119. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright notation thereon. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of 711 Human Performance Wing (HPW) and Defense Advanced Research Projects Agency (DARPA) or the U.S. Government. T.C.-K., S. Jo, and I.L. acknowledge support from Carnegie Mellon University Department of Materials Science and Engineering Materials Characterization Facility supported by Grant MCF-677785. T.C.-K., S. Jo, and I.L. would like to thank Dr. Loren Rieth for helpful discussion about SIROF and Mark Weiler and Dr. Matthew Moneck from the Claire and John Bertucci Nanotechnology Laboratory for assistance with SIROF deposition. J.R., A. Surendran, and X.J. acknowledge Northwestern University’s NUANCE Center, which has received support from the SHyNE Resource (NSF ECCS-2025633), the IIN, and Northwestern’s MRSEC program (NSF DMR-1720139). J.R. and A. Surendran acknowledge support from Analytical BioNanoTechnology Equipment Core (ANTEC) supported by the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-2025633) for material characterization and Center for Advanced Microscopy/Nikon Imaging Center (CAM) supported by CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center for confocal imaging. J.R., T.C.-K., A. Surendran, and I.L. acknowledge support from Blackrock Neurotech for SIROF, and wire-bundle bonding for the in vivo experiments. D.J.S. was supported by the National Heart, Lung, and Blood Institute of the National Institutes of Health under Award Number K99HL155777. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
ASJC Scopus subject areas
- General Chemistry
- General Biochemistry, Genetics and Molecular Biology
- General Physics and Astronomy