Electrochemical DNA-Based Immunoassay That Employs Steric Hindrance to Detect Small Molecules Directly in Whole Blood

Sahar S. Mahshid, Francesco Ricci, Shana O. Kelley, Alexis Vallée-Bélisle*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The development of a universal sensing mechanism for the rapid and quantitative detection of small molecules directly in whole blood would drastically impact global health by enabling disease diagnostics, monitoring, and treatment at home. We have previously shown that hybridization between a free DNA strand and its complementary surface-bound strand can be sterically hindered when the former is bound to large antibodies. Here, we exploit this effect to design a competitive antibody-based electrochemical assay, called CeSHHA, that enables the quantitative detection of small molecules directly in complex matrices, such as whole blood or soil. We discuss the importance of this inexpensive assay for point-of-care diagnosis and for treatment monitoring applications.

Original languageEnglish (US)
Pages (from-to)718-723
Number of pages6
JournalACS Sensors
Volume2
Issue number6
DOIs
StatePublished - Jun 23 2017
Externally publishedYes

Keywords

  • DNA nanotechnology
  • biosensors
  • point-of-care
  • therapeutic drug monitoring
  • whole blood

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Fluid Flow and Transfer Processes
  • Process Chemistry and Technology

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