Electrochemically controllable conjugation of proteins on surfaces

Paula M. Mendes*, Karen L. Christman, Puru Parthasarathy, Eric Schopf, Jianyong Ouyang, Yang Yang, Jon A. Preece, Heather D. Maynard, Yong Chen, J. Fraser Stoddart

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The rational design of surfaces for immobilization of proteins is essential to a variety of biological and medical applications ranging from molecular diagnostics to advanced platforms for fundamental studies of molecular and cell biology. We have developed an advanced electrochemically based approach for site-selective and reaction-controlled immobilization of proteins on surfaces. When a molecular monolayer of 4-nitrothiophenol on gold electrode surfaces is reduced electrochemically in a selective fashion at its nitro groups, to afford amino groups by potentiometric scans, the amine can be employed to orchestrate the immobilization of proteins to the surface. This protein immobilization strategy could allow one to fabricate intricate protein structures on surfaces for addressing fundamental and applied problems in biology and medicine.

Original languageEnglish (US)
Pages (from-to)1919-1923
Number of pages5
JournalBioconjugate Chemistry
Volume18
Issue number6
DOIs
StatePublished - Nov 1 2007

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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