Electron-nuclear double resonance in melanins

T. Sarna; C. Mailer; J. S. Hyde; H. M. Swartz; B. M. Hoffman

doi:10.1016/S0006-3495(76)85765-7

Electron-nuclear double resonance in melanins

T. Sarna^*, C. Mailer, J. S. Hyde, H. M. Swartz, B. M. Hoffman

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Electron-nuclear double resonance (ENDOR) signals from matrix protons interacting with the stable free radicals of "A"- and "B"-type melanins have been observed as a function of pH. In all samples the single line is similar in width and unusually narrow. The ENDOR reduction varies by more than a factor of 10, indicating a large sensitivity of relaxation properties of melanin to sample type. Signals were observed over a wide range of experimental conditions with good signal-to-noise ratio, establishing feasibility for further more detailed ENDOR studies. Incubation in D2O resulted in little change, indicating that the free radical is well buried or protected. No resolved hyperfine structure was seen, consistent with the generally accepted view that melanin is a heterogeneous polymer.

Original language	English (US)
Pages (from-to)	1165-1170
Number of pages	6
Journal	Biophysical Journal
Volume	16
Issue number	10
DOIs	https://doi.org/10.1016/S0006-3495(76)85765-7
State	Published - 1976

Funding

(BMS 00478), DA-ARO-D (31-124), and the donors to the Petroleum Research Fund administered by the American Chemical Society. Receivedforpublication I April 1976 and in revisedform8 June 1976. This work was supported in part by the John Hartford Foundation, the National Science Foundation

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(76)85765-7

Cite this

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title = "Electron-nuclear double resonance in melanins",

abstract = "Electron-nuclear double resonance (ENDOR) signals from matrix protons interacting with the stable free radicals of {"}A{"}- and {"}B{"}-type melanins have been observed as a function of pH. In all samples the single line is similar in width and unusually narrow. The ENDOR reduction varies by more than a factor of 10, indicating a large sensitivity of relaxation properties of melanin to sample type. Signals were observed over a wide range of experimental conditions with good signal-to-noise ratio, establishing feasibility for further more detailed ENDOR studies. Incubation in D2O resulted in little change, indicating that the free radical is well buried or protected. No resolved hyperfine structure was seen, consistent with the generally accepted view that melanin is a heterogeneous polymer.",

author = "T. Sarna and C. Mailer and Hyde, {J. S.} and Swartz, {H. M.} and Hoffman, {B. M.}",

note = "Funding Information: (BMS 00478), DA-ARO-D (31-124), and the donors to the Petroleum Research Fund administered by the American Chemical Society. Receivedforpublication I April 1976 and in revisedform8 June 1976. Funding Information: This work was supported in part by the John Hartford Foundation, the National Science Foundation",

year = "1976",

doi = "10.1016/S0006-3495(76)85765-7",

language = "English (US)",

volume = "16",

pages = "1165--1170",

journal = "Biophysical Journal",

issn = "0006-3495",

publisher = "Biophysical Society",

number = "10",

}

TY - JOUR

T1 - Electron-nuclear double resonance in melanins

AU - Sarna, T.

AU - Mailer, C.

AU - Hyde, J. S.

AU - Swartz, H. M.

AU - Hoffman, B. M.

N1 - Funding Information: (BMS 00478), DA-ARO-D (31-124), and the donors to the Petroleum Research Fund administered by the American Chemical Society. Receivedforpublication I April 1976 and in revisedform8 June 1976. Funding Information: This work was supported in part by the John Hartford Foundation, the National Science Foundation

PY - 1976

Y1 - 1976

N2 - Electron-nuclear double resonance (ENDOR) signals from matrix protons interacting with the stable free radicals of "A"- and "B"-type melanins have been observed as a function of pH. In all samples the single line is similar in width and unusually narrow. The ENDOR reduction varies by more than a factor of 10, indicating a large sensitivity of relaxation properties of melanin to sample type. Signals were observed over a wide range of experimental conditions with good signal-to-noise ratio, establishing feasibility for further more detailed ENDOR studies. Incubation in D2O resulted in little change, indicating that the free radical is well buried or protected. No resolved hyperfine structure was seen, consistent with the generally accepted view that melanin is a heterogeneous polymer.

AB - Electron-nuclear double resonance (ENDOR) signals from matrix protons interacting with the stable free radicals of "A"- and "B"-type melanins have been observed as a function of pH. In all samples the single line is similar in width and unusually narrow. The ENDOR reduction varies by more than a factor of 10, indicating a large sensitivity of relaxation properties of melanin to sample type. Signals were observed over a wide range of experimental conditions with good signal-to-noise ratio, establishing feasibility for further more detailed ENDOR studies. Incubation in D2O resulted in little change, indicating that the free radical is well buried or protected. No resolved hyperfine structure was seen, consistent with the generally accepted view that melanin is a heterogeneous polymer.

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DO - 10.1016/S0006-3495(76)85765-7

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AN - SCOPUS:0017131487

SN - 0006-3495

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SP - 1165

EP - 1170

JO - Biophysical Journal

JF - Biophysical Journal

IS - 10

ER -

Electron-nuclear double resonance in melanins

Abstract

Funding

ASJC Scopus subject areas

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