Electron-nuclear double resonance of copper complexes of human transferrin

James E. Roberts, Theodore G. Brown, Brian M. Hoffman, Philip Aisen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Electron-nuclear double resonance (ENDOR) spectroscopy has been used to study ligand and copper hyperfine interactions in Cu(II) complexes of human transferrin. A nearly isotropic superhyperfine interaction of the Cu(II) spin with a single 14N nucleus was identified, and the principal values of its tensor were estimated. All principal values of the copper hyperfine tensor were also directly measured for the first time. Resonances from at least two exchangeable protons were observed, but their origin could not be ascertained. At physiological pH, and in the presence of bicarbonate, ENDOR spectra of the two metal-binding sites were virtually indisinguishable.

Original languageEnglish (US)
Pages (from-to)49-54
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume747
Issue number1-2
DOIs
StatePublished - Sep 14 1983

Keywords

  • (Human serum)
  • Copper complex
  • ENDOR
  • Transferrin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Electron-nuclear double resonance of copper complexes of human transferrin'. Together they form a unique fingerprint.

Cite this